Protein Variants | Comment | Organism |
---|---|---|
D315A | complete loss of activity | Escherichia coli |
H186A | complete loss of activity | Escherichia coli |
H242A | complete loss of activity | Escherichia coli |
H59A | complete loss of activity | Escherichia coli |
H61A | complete loss of activity | Escherichia coli |
K146A | complete loss of activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
(S)-allantoin | Co2+-activated enzyme, pH 8.0, 25°C | Escherichia coli | |
6.2 | - |
(S)-allantoin | Mn2+-activated enzyme, pH 8.0, 25°C | Escherichia coli | |
7.3 | - |
(S)-allantoin | Ni2+-activated enzyme, pH 8.0, 25°C | Escherichia coli | |
9.6 | - |
(S)-allantoin | Zn2+-activated enzyme, pH 8.0, 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57150 | - |
gel filtration | Escherichia coli |
93600 | - |
gel filtration | Escherichia coli |
189500 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | A0A140NCJ5 | - |
- |
Renatured (Comment) | Organism |
---|---|
purified recombinant ALLase exhibits no activity but can be activated when preincubating with some metal ions before analyzing its activity. Renaturation follows in decreasing order Mn2+, Co2+, Zn2+, Ni2+, Cd2+, Mg2+ | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.3 | - |
Ni2+-activated enzyme, pH 8.0, 25°C | Escherichia coli |
2.6 | - |
Zn2+-activated enzyme, pH 8.0, 25°C | Escherichia coli |
8 | - |
Co2+-activated enzyme, pH 8.0, 25°C | Escherichia coli |
88.2 | - |
Mn2+-activated enzyme, pH 8.0, 25°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-allantoin + H2O | - |
Escherichia coli | allantoate | - |
? | |
additional information | no substrate: hydantoin, dihydrouracil, phthalimide, dihydroorotate, and 3-imonoisoindolinone | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | gel filtration chromatography reveals a mixture of monomers, dimers, and tetramers | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ALLase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
Mn2+-activited enzyme | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, comnplete loss of activity | Escherichia coli |