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Literature summary for 3.5.2.5 extracted from

  • Ho, Y.Y.; Hsieh, H.C.; Huang, C.Y.
    Biochemical characterization of allantoinase from Escherichia coli BL21 (2011), Protein J., 30, 384-394.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D315A complete loss of activity Escherichia coli
H186A complete loss of activity Escherichia coli
H242A complete loss of activity Escherichia coli
H59A complete loss of activity Escherichia coli
H61A complete loss of activity Escherichia coli
K146A complete loss of activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5
-
(S)-allantoin Co2+-activated enzyme, pH 8.0, 25°C Escherichia coli
6.2
-
(S)-allantoin Mn2+-activated enzyme, pH 8.0, 25°C Escherichia coli
7.3
-
(S)-allantoin Ni2+-activated enzyme, pH 8.0, 25°C Escherichia coli
9.6
-
(S)-allantoin Zn2+-activated enzyme, pH 8.0, 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57150
-
gel filtration Escherichia coli
93600
-
gel filtration Escherichia coli
189500
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A140NCJ5
-
-

Renatured (Commentary)

Renatured (Comment) Organism
purified recombinant ALLase exhibits no activity but can be activated when preincubating with some metal ions before analyzing its activity. Renaturation follows in decreasing order Mn2+, Co2+, Zn2+, Ni2+, Cd2+, Mg2+ Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
Ni2+-activated enzyme, pH 8.0, 25°C Escherichia coli
2.6
-
Zn2+-activated enzyme, pH 8.0, 25°C Escherichia coli
8
-
Co2+-activated enzyme, pH 8.0, 25°C Escherichia coli
88.2
-
Mn2+-activated enzyme, pH 8.0, 25°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-allantoin + H2O
-
Escherichia coli allantoate
-
?
additional information no substrate: hydantoin, dihydrouracil, phthalimide, dihydroorotate, and 3-imonoisoindolinone Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
More gel filtration chromatography reveals a mixture of monomers, dimers, and tetramers Escherichia coli

Synonyms

Synonyms Comment Organism
ALLase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
Mn2+-activited enzyme Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
30 min, comnplete loss of activity Escherichia coli