Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | inactive in absence of zinc | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D315N | no activity | Escherichia coli |
N94D | no activity | Escherichia coli |
N94V | no activity | Escherichia coli |
R215K | no significant effect on enzyme activity | Escherichia coli |
S288D | no significant effect on enzyme activity | Escherichia coli |
S288V | no significant effect on enzyme activity | Escherichia coli |
S317D | no activity | Escherichia coli |
S317V | no activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28.6 | - |
(S)-allantoin | wild-type allantoinase, supplementation with zinc acetate | Escherichia coli | |
37.5 | - |
(S)-allantoin | selenomethionine-substituted wild-type allantoinase, no supplementation with zinc acetate | Escherichia coli | |
53.8 | - |
(S)-allantoin | mutant S288D, supplementation with zinc acetate | Escherichia coli | |
62.8 | - |
(S)-allantoin | mutant R215K, supplementation with zinc acetate | Escherichia coli | |
91.3 | - |
(S)-allantoin | mutant S288V, supplementation with zinc acetate | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-allantoin + H2O | Escherichia coli | final step of ureide pathway | allantoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77671 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-allantoin + H2O | - |
Escherichia coli | allantoate | - |
? | |
(S)-allantoin + H2O | final step of ureide pathway | Escherichia coli | allantoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
allantoinase | - |
Escherichia coli |
Fe-allantoinase | - |
Escherichia coli |
metal-dependent allantoinase | - |
Escherichia coli |
zinc-amended allantoinase | - |
Escherichia coli |