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Literature summary for 3.5.2.5 extracted from

  • Mulrooney, S.B.; Hausinger, R.P.
    Metal ion dependence of recombinant Escherichia coli allantoinase (2003), J. Bacteriol., 185, 126-134.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.5

Cloned(Commentary)

Cloned (Comment) Organism
overproduction of enzyme in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol competitive Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17
-
 (S)-allantoin pH 7.4, 37°C, Zn2+-amended culture Escherichia coli
19.5
-
 (S)-allantoin pH 7.4, 37°C, Co2+-amended culture Escherichia coli
79.9
-
 (S)-allantoin pH 7.4, 37°C, Ni2+-amended culture Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
76.1
-
 pH 7.4, 37°C, Zn2+-amended culture Escherichia coli
410
-
 pH 7.4, 37°C, Co2+-amended culture Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-allantoin + H2O zinc enzyme uses only the S-isomer, cobalt-enzyme prefers S-isomer but also hydrolyses R-isomer Escherichia coli allantoate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.237
-
 dithiothreitol pH 7.4, 37°C, Co2+-amended culture Escherichia coli
0.795
-
 dithiothreitol pH 7.4, 37°C, Zn2+-amended culture Escherichia coli