Literature summary for 3.5.2.3 extracted from

Ogawa, J.; Shimizu, S.
Purification and characterization of dihydroorotase from Pseudomonas putida (1995), Arch. Microbiol., 164, 353-357.

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Inhibitors

Inhibitors	Comment	Organism	Structure
N-carbamoylamino acids	competitive inhibition	Pseudomonas putida
Orotate	noncompetitive inhibition	Pseudomonas putida
sulfhydryl reagents	-	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.081	-	L-dihydroorotate	-	Pseudomonas putida
2.2	-	L-carbamoylaspartate	-	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41000	-	2 * 41000 SDS-PAGE	Pseudomonas putida
82000	-	gel filtration	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-carbamoyl-L-aspartate	Pseudomonas putida	-	L-dihydroorotate + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
18	-	L-dihydroorotate as substrate	Pseudomonas putida
68	-	L-carbamoylaspartate as substrate	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-carbamoylaspartate	-	Pseudomonas putida	L-dihydroorotate + H2O	-	r
N-carbamoyl-L-aspartate	-	Pseudomonas putida	L-dihydroorotate + H2O	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 41000 SDS-PAGE	Pseudomonas putida

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Release 2023.1 (January 2023)