KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
isatin | pH 9.0, 32°C | Mesorhizobium loti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isatin + H2O | Bradyrhizobium japonicum | the enzyme is involved in the degradation of indole-3-acetic acid | isatinate | - |
? | |
isatin + H2O | Mesorhizobium loti | the enzyme is involved in the degradation of indole-3-acetic acid | isatinate | - |
? | |
isatin + H2O | Neorhizobium galegae | the enzyme is involved in the degradation of indole-3-acetic acid | isatinate | - |
? | |
isatin + H2O | Bradyrhizobium japonicum USDA122 | the enzyme is involved in the degradation of indole-3-acetic acid | isatinate | - |
? | |
isatin + H2O | Bradyrhizobium japonicum 61A24 | the enzyme is involved in the degradation of indole-3-acetic acid | isatinate | - |
? | |
isatin + H2O | Mesorhizobium loti NZP 2037 | the enzyme is involved in the degradation of indole-3-acetic acid | isatinate | - |
? | |
isatin + H2O | Bradyrhizobium japonicum 110 | the enzyme is involved in the degradation of indole-3-acetic acid | isatinate | - |
? | |
isatin + H2O | Neorhizobium galegae HAMBI 540 | the enzyme is involved in the degradation of indole-3-acetic acid | isatinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | - |
- |
- |
Bradyrhizobium japonicum 110 | - |
- |
- |
Bradyrhizobium japonicum 61A24 | - |
- |
- |
Bradyrhizobium japonicum USDA122 | - |
- |
- |
Mesorhizobium loti | - |
- |
- |
Mesorhizobium loti NZP 2037 | - |
- |
- |
Neorhizobium galegae | - |
- |
- |
Neorhizobium galegae HAMBI 540 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Mesorhizobium loti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.34 | - |
pH 9.0, 32°C | Bradyrhizobium japonicum |
0.41 | - |
pH 9.0, 32°C | Bradyrhizobium japonicum |
0.611 | - |
pH 9.0, 32°C | Bradyrhizobium japonicum |
0.64 | - |
pH 9.0, 32°C | Mesorhizobium loti |
0.69 | - |
pH 9.0, 32°C | Neorhizobium galegae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isatin + H2O | the enzyme is involved in the degradation of indole-3-acetic acid | Bradyrhizobium japonicum | isatinate | - |
? | |
isatin + H2O | the enzyme is involved in the degradation of indole-3-acetic acid | Mesorhizobium loti | isatinate | - |
? | |
isatin + H2O | the enzyme is involved in the degradation of indole-3-acetic acid | Neorhizobium galegae | isatinate | - |
? | |
isatin + H2O | isatin i.e. 1H-indole-2,3-dione | Bradyrhizobium japonicum | isatinate | isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate | ? | |
isatin + H2O | isatin i.e. 1H-indole-2,3-dione | Mesorhizobium loti | isatinate | isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate | ? | |
isatin + H2O | isatin i.e. 1H-indole-2,3-dione | Neorhizobium galegae | isatinate | isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate | ? | |
isatin + H2O | the enzyme is involved in the degradation of indole-3-acetic acid | Bradyrhizobium japonicum USDA122 | isatinate | - |
? | |
isatin + H2O | isatin i.e. 1H-indole-2,3-dione | Bradyrhizobium japonicum USDA122 | isatinate | isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate | ? | |
isatin + H2O | the enzyme is involved in the degradation of indole-3-acetic acid | Bradyrhizobium japonicum 61A24 | isatinate | - |
? | |
isatin + H2O | isatin i.e. 1H-indole-2,3-dione | Bradyrhizobium japonicum 61A24 | isatinate | isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate | ? | |
isatin + H2O | the enzyme is involved in the degradation of indole-3-acetic acid | Mesorhizobium loti NZP 2037 | isatinate | - |
? | |
isatin + H2O | isatin i.e. 1H-indole-2,3-dione | Mesorhizobium loti NZP 2037 | isatinate | isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate | ? | |
isatin + H2O | the enzyme is involved in the degradation of indole-3-acetic acid | Bradyrhizobium japonicum 110 | isatinate | - |
? | |
isatin + H2O | isatin i.e. 1H-indole-2,3-dione | Bradyrhizobium japonicum 110 | isatinate | isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate | ? | |
isatin + H2O | the enzyme is involved in the degradation of indole-3-acetic acid | Neorhizobium galegae HAMBI 540 | isatinate | - |
? | |
isatin + H2O | isatin i.e. 1H-indole-2,3-dione | Neorhizobium galegae HAMBI 540 | isatinate | isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate | ? |
Synonyms | Comment | Organism |
---|---|---|
isatin amidohydrolase | - |
Bradyrhizobium japonicum |
isatin amidohydrolase | - |
Mesorhizobium loti |
isatin amidohydrolase | - |
Neorhizobium galegae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
assay at | Bradyrhizobium japonicum |
32 | - |
assay at | Mesorhizobium loti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Bradyrhizobium japonicum |
9 | - |
assay at | Mesorhizobium loti |
Organism | Comment | Expression |
---|---|---|
Mesorhizobium loti | the enzyme is inducible (4-5-fold) by its substrate, isatin | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the degradation of indole-3-acetic acid | Bradyrhizobium japonicum |
metabolism | the enzyme is involved in the degradation of indole-3-acetic acid | Mesorhizobium loti |
metabolism | the enzyme is involved in the degradation of indole-3-acetic acid | Neorhizobium galegae |