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Literature summary for 3.5.2.2 extracted from

  • Lee, S.; Chang, Y.; Shin, D.; Han, J.; Seo, M.; Fazelinia, H.; Maranas, C.; Kim, H.
    Designing the substrate specificity of D-hydantoinase using a rational approach (2009), Enzyme Microb. Technol., 44, 170-175.
No PubMed abstract available
Search for more literature for 3.5.2.2

Protein Variants

Protein Variants Comment Organism
F159S 400% of wild-type activity Geobacillus stearothermophilus
M63A 120% of wild-type activity Geobacillus stearothermophilus
M63A/F159S 411% of wild-type activity Geobacillus stearothermophilus
M63H/F159N 450% of wild-type activity Geobacillus stearothermophilus
M63H/F159R 168% of wild-type activity Geobacillus stearothermophilus
M63H/F159S 353% of wild-type activity Geobacillus stearothermophilus
M63I 108% of wild-type activity Geobacillus stearothermophilus
M63I/F159A 374% of wild-type activity Geobacillus stearothermophilus
M63I/F159S 540% of wild-type activity Geobacillus stearothermophilus
M63I/F159T 411% of wild-type activity Geobacillus stearothermophilus
M63Q/F159N 213% of wild-type activity Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
 DL-p-hydroxyphenylhydantoin mutant M63I/F159S, pH 8.0, 37°C Geobacillus stearothermophilus
4.9
-
 DL-p-hydroxyphenylhydantoin wild-type, pH 8.0, 37°C Geobacillus stearothermophilus
5.1
-
 DL-p-hydroxyphenylhydantoin mutant M63H/F159N, pH 8.0, 37°C Geobacillus stearothermophilus
78
-
 hydantoin wild-type, pH 8.0, 37°C Geobacillus stearothermophilus
382
-
 hydantoin mutant M63I/F159S, pH 8.0, 37°C Geobacillus stearothermophilus
416
-
 hydantoin mutant M63H/F159N, pH 8.0, 37°C Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q45515
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-p-hydroxyphenylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-hydroxyphenylglycine
-
 ?
hydantoin + H2O
-
 Geobacillus stearothermophilus hydantoic acid
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
 hydantoin mutant M63I/F159S, pH 8.0, 37°C Geobacillus stearothermophilus
18
-
 DL-p-hydroxyphenylhydantoin wild-type, pH 8.0, 37°C Geobacillus stearothermophilus
57
-
 hydantoin mutant M63H/F159N, pH 8.0, 37°C Geobacillus stearothermophilus
59
-
 hydantoin wild-type, pH 8.0, 37°C Geobacillus stearothermophilus
59
-
 DL-p-hydroxyphenylhydantoin mutant M63I/F159S, pH 8.0, 37°C Geobacillus stearothermophilus
62
-
 DL-p-hydroxyphenylhydantoin mutant M63H/F159N, pH 8.0, 37°C Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.022
-
 hydantoin mutant M63I/F159S, pH 8.0, 37°C Geobacillus stearothermophilus
0.137
-
 hydantoin mutant M63H/F159N, pH 8.0, 37°C Geobacillus stearothermophilus
0.763
-
 hydantoin wild-type, pH 8.0, 37°C Geobacillus stearothermophilus
3.63
-
 DL-p-hydroxyphenylhydantoin wild-type, pH 8.0, 37°C Geobacillus stearothermophilus
12.17
-
 DL-p-hydroxyphenylhydantoin mutant M63H/F159N, pH 8.0, 37°C Geobacillus stearothermophilus
20.95
-
 DL-p-hydroxyphenylhydantoin mutant M63I/F159S, pH 8.0, 37°C Geobacillus stearothermophilus