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Literature summary for 3.5.2.2 extracted from

  • Morin, A.; Leblanc, D.; Paleczek, A.; Hummel, W.; Kula, M.R.
    Comparison of seven microbial D-hydantoinases (1990), J. Biotechnol., 16, 37-48.
No PubMed abstract available
Search for more literature for 3.5.2.2

Application

Application Comment Organism
nutrition
-
 Pseudomonas fluorescens
nutrition
-
 Pseudomonas putida
nutrition
-
 Serratia liquefaciens
nutrition
-
 Corynebacterium pseudodiphtheriticum

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Corynebacterium pseudodiphtheriticum
190000
-
-
 Pseudomonas putida
190000
-
 strain 2.2, gel filtration Pseudomonas putida
205000
-
 strain 2.5, gel filtration Pseudomonas putida
207000
-
 gel filtration Serratia liquefaciens
232000
-
 strain DSM 84, gel filtration Pseudomonas putida
235000
-
 strain 1.2 and 1.9, gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,6-dihydrouracil + H2O Pseudomonas fluorescens
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Serratia liquefaciens
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Corynebacterium pseudodiphtheriticum
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Serratia liquefaciens 1.15
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Corynebacterium pseudodiphtheriticum 14.10
-
 3-ureidopropionate
-
 ?
hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Serratia liquefaciens
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Corynebacterium pseudodiphtheriticum
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Serratia liquefaciens 1.15
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Corynebacterium pseudodiphtheriticum 14.10
-
 N-carbamoyl glycine
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium pseudodiphtheriticum
-
-
-
Corynebacterium pseudodiphtheriticum 14.10
-
-
-
Pseudomonas fluorescens
-
 strains 1.2 and 1.9
-
Pseudomonas putida
-
-
-
Pseudomonas putida
-
 strains 2.2, 2.5
-
Pseudomonas putida
-
 DSM 84, formerly classified as P. fluorescens
-
Serratia liquefaciens
-
-
-
Serratia liquefaciens 1.15
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida
-
 Serratia liquefaciens
-
 Corynebacterium pseudodiphtheriticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrouracil + H2O
-
 Pseudomonas fluorescens 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Serratia liquefaciens 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Corynebacterium pseudodiphtheriticum 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Serratia liquefaciens 1.15 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Corynebacterium pseudodiphtheriticum 14.10 3-ureidopropionate
-
 ?
hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Serratia liquefaciens N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Corynebacterium pseudodiphtheriticum N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Serratia liquefaciens 1.15 N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Corynebacterium pseudodiphtheriticum 14.10 N-carbamoyl glycine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45 strain 2.5 Pseudomonas putida
45 55
-
 Serratia liquefaciens
45 55
-
 Corynebacterium pseudodiphtheriticum
45 55 strain 2.2 and 84 Pseudomonas putida
45 55 strain 1.2 Pseudomonas fluorescens
57
-
 strain 14.10 Corynebacterium pseudodiphtheriticum
57
-
 strain 1.9 Pseudomonas fluorescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 60
-
 Pseudomonas fluorescens
22 60
-
 Pseudomonas putida
22 60
-
 Serratia liquefaciens
22 60
-
 Corynebacterium pseudodiphtheriticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 strain 2.2 and 84, stable activity for at least 50 h Pseudomonas putida
37
-
 stable up to at pH 5 Pseudomonas fluorescens
37
-
 stable up to at pH 5 Serratia liquefaciens
37
-
 strain 2.5 Pseudomonas putida
45
-
 strains 1.2 and 1.9 Pseudomonas fluorescens
57
-
 stable up to at above pH 8 Corynebacterium pseudodiphtheriticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas fluorescens
8
-
-
 Serratia liquefaciens
8
-
 strain 84 Pseudomonas putida
10
-
-
 Corynebacterium pseudodiphtheriticum

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Pseudomonas fluorescens
6 10
-
 Pseudomonas putida
6 10
-
 Serratia liquefaciens
6 10
-
 Corynebacterium pseudodiphtheriticum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 strain 2.2, other strains more stable around pH 5 Pseudomonas putida
5
-
-
 Corynebacterium pseudodiphtheriticum
5
-
 strain 84 Pseudomonas putida
5.5 8.5
-
 Pseudomonas fluorescens
5.5 8.5
-
 Pseudomonas putida