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Literature summary for 3.5.2.2 extracted from
- Microbial synthesis of D-amino acids from DL-5-substituted hydantoins (1983), Hakko Kogaku Kaishi, 61, 139-151.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | - |
Pseudomonas putida |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | - |
Pseudomonas putida |  |
| o-phenanthroline | - |
Pseudomonas putida | |
| p-hydroxymercuribenzoate | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
5,6-dihydrouracil | - |
Pseudomonas putida | |
| 34 | - |
DL-5-(2-methylthioethyl)-hydantoin | - |
Pseudomonas putida | |
| 50 | - |
DL-5-phenylhydantoin | - |
Pseudomonas putida | |
| 350 | - |
hydantoin | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydropyrimidine + H2O | Mycolicibacterium smegmatis | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Enterobacter cloacae | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Pseudomonas putida | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Streptomyces griseus | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Delftia acidovorans | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Streptomyces albus | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Gordonia rubripertincta | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Pseudomonas chlororaphis | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Clavibacter michiganensis subsp. sepedonicus | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Streptomyces mitakaensis | - |
beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | Actinoplanes philippinensis | - |
beta-ureido acid | - |
? | |
| 5,6-dihydrouracil + H2O | Mycolicibacterium smegmatis | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Enterobacter cloacae | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Pseudomonas putida | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Streptomyces griseus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Delftia acidovorans | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Streptomyces albus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Gordonia rubripertincta | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Agrobacterium rhizogenes | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Agrobacterium tumefaciens | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Pseudomonas chlororaphis | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Clavibacter michiganensis subsp. sepedonicus | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Streptomyces mitakaensis | - |
3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | Actinoplanes philippinensis | - |
3-ureidopropionate | - |
? | |
| hydantoin + H2O | Mycolicibacterium smegmatis | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Enterobacter cloacae | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Streptomyces griseus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Delftia acidovorans | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Streptomyces albus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Gordonia rubripertincta | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium rhizogenes | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Agrobacterium tumefaciens | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas chlororaphis | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Clavibacter michiganensis subsp. sepedonicus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Streptomyces mitakaensis | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Actinoplanes philippinensis | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinoplanes philippinensis | - |
- |
- |
| Agrobacterium rhizogenes | - |
- |
- |
| Agrobacterium tumefaciens | - |
- |
- |
| Clavibacter michiganensis subsp. sepedonicus | - |
- |
- |
| Delftia acidovorans | - |
- |
- |
| Enterobacter cloacae | - |
- |
- |
| Gordonia rubripertincta | - |
- |
- |
| Mycolicibacterium smegmatis | - |
- |
- |
| Pseudomonas chlororaphis | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
| Streptomyces albus | - |
- |
- |
| Streptomyces griseus | - |
- |
- |
| Streptomyces mitakaensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydropyrimidine + H2O | - |
Mycolicibacterium smegmatis | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Enterobacter cloacae | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Pseudomonas putida | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Streptomyces griseus | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Delftia acidovorans | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Streptomyces albus | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Gordonia rubripertincta | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Pseudomonas chlororaphis | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Clavibacter michiganensis subsp. sepedonicus | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Streptomyces mitakaensis | beta-ureido acid | - |
? | |
| 5,6-dihydropyrimidine + H2O | - |
Actinoplanes philippinensis | beta-ureido acid | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Mycolicibacterium smegmatis | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Enterobacter cloacae | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas putida | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Streptomyces griseus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Delftia acidovorans | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Streptomyces albus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Gordonia rubripertincta | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium rhizogenes | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Agrobacterium tumefaciens | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas chlororaphis | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Clavibacter michiganensis subsp. sepedonicus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Streptomyces mitakaensis | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Actinoplanes philippinensis | 3-ureidopropionate | - |
? | |
| 5-(2-thienyl)-DL-hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-2-thienylglycine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Mycolicibacterium smegmatis | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Enterobacter cloacae | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Streptomyces griseus | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Delftia acidovorans | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Streptomyces albus | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Gordonia rubripertincta | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Pseudomonas chlororaphis | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Clavibacter michiganensis subsp. sepedonicus | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Streptomyces mitakaensis | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(2-methylthioethyl)-hydantoin + H2O | - |
Actinoplanes philippinensis | N-carbamoyl-D-methionine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Enterobacter cloacae | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-(p-hydroxyphenyl)hydantoin + H2O | - |
Clavibacter michiganensis subsp. sepedonicus | N-carbamoyl-p-hydroxyphenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl-D-phenylglycine | - |
? | |
| DL-5-phenylhydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl-D-phenylglycine | - |
? | |
| hydantoin + H2O | - |
Mycolicibacterium smegmatis | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Enterobacter cloacae | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Streptomyces griseus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Delftia acidovorans | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Streptomyces albus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Gordonia rubripertincta | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium rhizogenes | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Agrobacterium tumefaciens | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas chlororaphis | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Clavibacter michiganensis subsp. sepedonicus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Streptomyces mitakaensis | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Actinoplanes philippinensis | N-carbamoyl glycine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
DL-5-phenylhydantoin or hydantoin as substrate | Pseudomonas putida |
| 55 | - |
dihydrouracil or DL-5-(2-methylthioethyl)-hydantoin as substrate | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.3 | DL-5-phenylhydantoin or hydantoin as substrate | Pseudomonas putida |
| 8.3 | 8.5 | DL-5-(2-methylthioethyl)-hydantoin as substrate | Pseudomonas putida |
| 8.8 | 9 | dihydrouracil as substrate | Pseudomonas putida |
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