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Literature summary for 3.5.2.17 extracted from
- A familiar motif in a new context: the catalytic mechanism of hydroxyisourate hydrolase (2003), Biochemistry, 42, 6848-6852.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged enzyme expessed in Escherichia coli BL21 (DE3)pLysS | Glycine max |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E199A | activity is reduced 400fold relative to wild-type | Glycine max |
| E408A | mutant with no activity | Glycine max |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
5,6-diaminouracil | pH 7.5, 30°C | Glycine max |  |
| 11 | - |
5-hydroxyisourate | pH 7.5, 30°C | Glycine max | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxyisourate + H2O | Glycine max | - |
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
| 5-hydroxyisourate + H2O | Glycine max | ureide pathway | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
| Glycine max | - |
soybean | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-diaminouracil + H2O | slow alternative substrate | Glycine max | ? | - |
? | |
| 5-hydroxyisourate + H2O | - |
Glycine max | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
| 5-hydroxyisourate + H2O | ureide pathway | Glycine max | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? | |
| additional information | alloxan is no substrate | Glycine max | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HIUHase | - |
Glycine max |
| hydroxyisourate hydrolase | - |
Glycine max |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
5-hydroxyisourate | pH 7.5, 30°C | Glycine max | |
| 0.21 | - |
5,6-diaminouracil | pH 7.5, 30°C | Glycine max | |
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