Protein Variants | Comment | Organism |
---|---|---|
C7G/C108G | decrease in absorbance at 500 nm by 12.8%. Less than 10% of wild-type activity | Pseudomonas putida |
H151G | 120% of wild-type activity | Pseudomonas putida |
H247A | decrease in absorbance at 500 nm by 32.4%. Less than 10% of wild-type activity | Pseudomonas putida |
H86A | decrease in absorbance at 500 nm by 14.7%. Less than 10% of wild-type activity | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 5 mM, 9.4% residual activity | Pseudomonas putida | |
EDTA | 5 mM, 43.6% residual activity | Pseudomonas putida | |
Pyridine-2,4-dicarboxylate | 5 mM, 20.8% residual activity | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cobalt | purified imidase, less the addition of any metals, reveals 0.514 mol zinc, 0.102 mol cobalt, and 0.179 mol nickel per subunit | Pseudomonas putida | |
Nickel | purified imidase, less the addition of any metals, reveals 0.514 mol zinc, 0.102 mol cobalt, and 0.179 mol nickel per subunit | Pseudomonas putida | |
Zinc | purified imidase, less the addition of any metals, reveals 0.514 mol zinc, 0.102 mol cobalt, and 0.179 mol nickel per subunit. Enzyme purified from cells grown in zinc-supplemented medium contain an approximate stoichiometric amount of the metal of 1.65 mol zinc per subunit. The activity of the apo-imidase depleted of zinc is recovered with the addition of zinc in the lower concentration of 0-20 micorM, whereas the enzymatic activity is decreased in the presence of high concentration of zinc above 100 microM | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida YZ-26 | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
the activity of the apo-imidase depleted of zinc is recovered with the addition of zinc in the lower concentration of 0-20 micorM, whereas the enzymatic activity is decreased in the presence of high concentration of zinc above 100 microM | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-5-hydantoin + H2O | - |
Pseudomonas putida | hydantoic acid monoamide | - |
? | |
DL-5-hydantoin + H2O | - |
Pseudomonas putida YZ-26 | hydantoic acid monoamide | - |
? |