Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heavy metal ions | - |
Blastobacter sp. A17p-4 | |
sulfhydryl reagents | inhibition may be caused by modification of sulfhydryl residues involved in activity | Blastobacter sp. A17p-4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
Maleimide | - |
Blastobacter sp. A17p-4 | |
0.94 | - |
succinimide | - |
Blastobacter sp. A17p-4 | |
4.5 | - |
glutarimide | - |
Blastobacter sp. A17p-4 | |
5.7 | - |
hydantoin | - |
Blastobacter sp. A17p-4 | |
9.4 | - |
beta-ureidopropionate | - |
Blastobacter sp. A17p-4 | |
23 | - |
thiohydantoin | - |
Blastobacter sp. A17p-4 | |
31 | - |
rhodanine | - |
Blastobacter sp. A17p-4 | |
33 | - |
2,4-Thiazolidinedione | - |
Blastobacter sp. A17p-4 | |
52 | - |
dihydrouracil | - |
Blastobacter sp. A17p-4 | |
62 | - |
parabanic acid | - |
Blastobacter sp. A17p-4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
3 * 35000, SDS-PAGE | Blastobacter sp. A17p-4 |
105000 | - |
gel filtration | Blastobacter sp. A17p-4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrothymine + H2O | Blastobacter sp. A17p-4 | - |
? | - |
? | |
dihydrouracil + H2O | Blastobacter sp. A17p-4 | - |
N-carbamoyl-beta-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastobacter sp. A17p-4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Blastobacter sp. A17p-4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
927 | - |
- |
Blastobacter sp. A17p-4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-thiazolidinedione + H2O | - |
Blastobacter sp. A17p-4 | ? | - |
? | |
dihydrothymine + H2O | - |
Blastobacter sp. A17p-4 | ? | - |
? | |
dihydrouracil + H2O | - |
Blastobacter sp. A17p-4 | N-carbamoyl-beta-alanine | - |
? | |
glutarimide + H2O | - |
Blastobacter sp. A17p-4 | glutaric acid monoamide | - |
? | |
hydantoin + H2O | - |
Blastobacter sp. A17p-4 | hydantoic acid monoamide | - |
r | |
maleimide + H2O | - |
Blastobacter sp. A17p-4 | maleic acid monoamide | - |
? | |
parabanic acid + H2O | - |
Blastobacter sp. A17p-4 | ? | - |
? | |
rhodanine + H2O | - |
Blastobacter sp. A17p-4 | ? | - |
? | |
succinimide + H2O | - |
Blastobacter sp. A17p-4 | succinamic acid monoamide | - |
r | |
thiohydantoin + H2O | - |
Blastobacter sp. A17p-4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 35000, SDS-PAGE | Blastobacter sp. A17p-4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Blastobacter sp. A17p-4 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | more than 90% initial activity after incubation at 20-60°C for 30 min at pH 7.5, activity completely lost at 70°C | Blastobacter sp. A17p-4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | different pH profile for cyclic ureide and cyclic imide hydrolysis | Blastobacter sp. A17p-4 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | more than 80% initial activity after incubation at 30°C for 30 min | Blastobacter sp. A17p-4 |