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Literature summary for 3.5.2.16 extracted from

  • Ogawa, J.; Soong, C.L.; Honda, M.; Shimizu, S.
    Imidase, a dihydropyrimidinase-like enzyme involved in the metabolism of cyclic imides (1997), Eur. J. Biochem., 243, 322-327.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
heavy metal ions
-
Blastobacter sp. A17p-4
sulfhydryl reagents inhibition may be caused by modification of sulfhydryl residues involved in activity Blastobacter sp. A17p-4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
Maleimide
-
Blastobacter sp. A17p-4
0.94
-
succinimide
-
Blastobacter sp. A17p-4
4.5
-
glutarimide
-
Blastobacter sp. A17p-4
5.7
-
hydantoin
-
Blastobacter sp. A17p-4
9.4
-
beta-ureidopropionate
-
Blastobacter sp. A17p-4
23
-
thiohydantoin
-
Blastobacter sp. A17p-4
31
-
rhodanine
-
Blastobacter sp. A17p-4
33
-
2,4-Thiazolidinedione
-
Blastobacter sp. A17p-4
52
-
dihydrouracil
-
Blastobacter sp. A17p-4
62
-
parabanic acid
-
Blastobacter sp. A17p-4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
3 * 35000, SDS-PAGE Blastobacter sp. A17p-4
105000
-
gel filtration Blastobacter sp. A17p-4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrothymine + H2O Blastobacter sp. A17p-4
-
?
-
?
dihydrouracil + H2O Blastobacter sp. A17p-4
-
N-carbamoyl-beta-alanine
-
?

Organism

Organism UniProt Comment Textmining
Blastobacter sp. A17p-4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Blastobacter sp. A17p-4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
927
-
-
Blastobacter sp. A17p-4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-thiazolidinedione + H2O
-
Blastobacter sp. A17p-4 ?
-
?
dihydrothymine + H2O
-
Blastobacter sp. A17p-4 ?
-
?
dihydrouracil + H2O
-
Blastobacter sp. A17p-4 N-carbamoyl-beta-alanine
-
?
glutarimide + H2O
-
Blastobacter sp. A17p-4 glutaric acid monoamide
-
?
hydantoin + H2O
-
Blastobacter sp. A17p-4 hydantoic acid monoamide
-
r
maleimide + H2O
-
Blastobacter sp. A17p-4 maleic acid monoamide
-
?
parabanic acid + H2O
-
Blastobacter sp. A17p-4 ?
-
?
rhodanine + H2O
-
Blastobacter sp. A17p-4 ?
-
?
succinimide + H2O
-
Blastobacter sp. A17p-4 succinamic acid monoamide
-
r
thiohydantoin + H2O
-
Blastobacter sp. A17p-4 ?
-
?

Subunits

Subunits Comment Organism
trimer 3 * 35000, SDS-PAGE Blastobacter sp. A17p-4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Blastobacter sp. A17p-4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 more than 90% initial activity after incubation at 20-60°C for 30 min at pH 7.5, activity completely lost at 70°C Blastobacter sp. A17p-4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 8.5 different pH profile for cyclic ureide and cyclic imide hydrolysis Blastobacter sp. A17p-4

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 more than 80% initial activity after incubation at 30°C for 30 min Blastobacter sp. A17p-4