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Literature summary for 3.5.2.16 extracted from
- Identification, purification, and characterization of a thermophilic imidase from pig liver (2000), Protein Expr. Purif., 19, 289-297.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
Dihydrothymine | pH 9, 60°C | Sus scrofa |  |
| 7.7 | - |
Phthalimide | pH 7, 60°C, KM increases about 20-30fold when the reaction temperature increases from 25 to 60°C | Sus scrofa | |
| 10.1 | - |
hydantoin | pH 9, 60°C, KM increases about 20-30fold when the reaction temperature increases from 25 to 60°C | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
6 * 51000, SDS-PAGE | Sus scrofa |
| 300000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrothymine + H2O | Sus scrofa | - |
? | - |
? | |
| dihydrouracil + H2O | Sus scrofa | - |
N-carbamoyl-beta-alanine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
thermophilic enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| salt precipitation, octyl Sepharose, DEAE-Sephacel, chelating Sephacel, gel filtration | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.3 | - |
measured at 50°C, increasing the reaction temperature from 25 to 60°C leads to an 50 and 60fold increase in specific activity for dihydrouracil and dihydrothymine, respectively, similar activities are found in other mammals | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrothymine + H2O | - |
Sus scrofa | ? | - |
? | |
| dihydrouracil + H2O | - |
Sus scrofa | N-carbamoyl-beta-alanine | - |
? | |
| hydantoin + H2O | - |
Sus scrofa | hydantoic acid monoamide | - |
? | |
| phtalimide + H2O | - |
Sus scrofa | 2-(aminocarbonyl)benzoic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 51000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Sus scrofa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | imidase acitivity increases with the elevation of temperature except for a reaction at pH 6 | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | at a pH around 7.5 and 8.5, imidase is stable at 50°C or at lower temperature for over 2 h, no activity above 70°C | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | shifts to more acidic pH at higher temperatures | Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | below 40°C, less stable at 50 and 60°C, stability decreases in acidic or alkaline pH at 60°C | Sus scrofa |
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