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Literature summary for 3.5.2.15 extracted from

  • Seffernick, J.L.; Erickson, J.S.; Cameron, S.; Cho, S.; Dodge, A.G.; Richman, J.; Sadowsky, M.J.; Wackett, L.P.
    Defining the cyanuric acid hydrolase (AtzD)/barbiturase protein family: Sequences and Reactions (2012), J. Bacteriol., 194, 4579-4588.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.15

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Moorella thermoacetica
expression in Escherichia coli Acidovorax citrulli
expression in Escherichia coli Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 cyanuric acid pH 8.0, 22°C Pseudomonas sp.
0.058
-
 cyanuric acid pH 8.0, 22°C Acidovorax citrulli
0.11
-
 cyanuric acid pH 8.0, 22°C Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Acidovorax citrulli
-
-
-
Acidovorax citrulli 12227
-
-
-
Moorella thermoacetica
-
-
-
Pseudomonas sp. P58329
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanuric acid + H2O
-
 Moorella thermoacetica carboxybiuret product spontaneously decarboxylates to give biuret and CO2. Therefore cyanuric acid hydrolase and barbiturase catalyze completely analogous reactions ?
cyanuric acid + H2O
-
 Acidovorax citrulli carboxybiuret product spontaneously decarboxylates to give biuret and CO2. Therefore cyanuric acid hydrolase and barbiturase catalyze completely analogous reactions ?
cyanuric acid + H2O
-
 Pseudomonas sp. carboxybiuret product spontaneously decarboxylates to give biuret and CO2. Therefore cyanuric acid hydrolase and barbiturase catalyze completely analogous reactions ?
cyanuric acid + H2O
-
 Acidovorax citrulli 12227 carboxybiuret product spontaneously decarboxylates to give biuret and CO2. Therefore cyanuric acid hydrolase and barbiturase catalyze completely analogous reactions ?

Synonyms

Synonyms Comment Organism
AtzD
-
 Pseudomonas sp.
TrzD
-
 Acidovorax citrulli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.6
-
 cyanuric acid pH 8.0, 22°C Moorella thermoacetica
14.2
-
 cyanuric acid pH 8.0, 22°C Acidovorax citrulli
73
-
 cyanuric acid pH 8.0, 22°C Pseudomonas sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
100
-
 cyanuric acid pH 8.0, 22°C Moorella thermoacetica
2500
-
 cyanuric acid pH 8.0, 22°C Acidovorax citrulli
3200
-
 cyanuric acid pH 8.0, 22°C Pseudomonas sp.