Any feedback?
Literature summary for 3.5.2.14 extracted from
- Nucleoside triphosphatase activity of an ATP-dependent enzyme, N-methylhydantoin amidohydrolase (1995), Biosci. Biotechnol. Biochem., 59, 1737-1739.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-methylhydantoin | inhibits the hydrolysis of other nucleoside triphosphates than ATP and dATP | Pseudomonas putida |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Pseudomonas putida | |
| Mg2+ | - |
Pseudomonas putida | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
77 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| CTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| dATP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| dATP + N-methylimidazolidine-2,4-dione + H2O | - |
Pseudomonas putida | dADP + phosphate + N-carbamoylsarcosine | - |
? | |
| dGTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| dITP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| dUTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| GTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| ITP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| UTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
| XTP + dihydrouracil + H2O | - |
Pseudomonas putida | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
ATP in presence of N-methylhydantoin | Pseudomonas putida |
| 50 | - |
CTP in absence of N-methylhydantoin | Pseudomonas putida |
| 55 | - |
CTP in presence of N-methylhydantoin | Pseudomonas putida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 70 | CTP-hydrolysis | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
about 70% of initial activity remains | Pseudomonas putida |
| 70 | - |
about 20% of initial activity remains | Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9 | - |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
loss of 25% in activity | Pseudomonas putida |
| 10.6 | - |
loss of 30% in activity | Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
