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Literature summary for 3.5.2.10 extracted from

  • Sakslund, H.; Hammerich, O.
    Effects of pH, temperature and reaction products on the performance of an immobilized creatininase-creatinase-sarcosine oxidase enzyme system for creatinine determination (1992), Anal. Chim. Acta, 268, 331-345.
No PubMed abstract available
Search for more literature for 3.5.2.10

Activating Compound

Activating Compound Comment Organism Structure
glycine
-
 Flavobacterium sp.

General Stability

General Stability Organism
enzyme immobilized have a long-term stability, unused 6 month Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
formaldehyde
-
 Flavobacterium sp.
methanol
-
 Flavobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
creatinine + H2O Flavobacterium sp. creatine metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
 Sigma, Cat. No.C-7399, lot 20 Ho349
-

Storage Stability

Storage Stability Organism
4°C, 0.1 M phosphate buffer, pH 7.7, enzymes controlled-pore glass immobilized Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
creatinine + H2O
-
 Flavobacterium sp. creatine
-
 r
creatinine + H2O creatine metabolism Flavobacterium sp. ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 immobilized coupled enzyme system Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 immobilized coupled enzyme system Flavobacterium sp.
8
-
 immobilized Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 immobilized Flavobacterium sp.