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Literature summary for 3.5.1.B15 extracted from

  • Ramirez-Busby, S.M.; Valafar, F.
    Systematic review of mutations in pyrazinamidase associated with pyrazinamide resistance in Mycobacterium tuberculosis clinical isolates (2015), Antimicrob. Agents Chemother., 59, 5267-5277 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.B15

Application

Application Comment Organism
diagnostics the pyrazinamidase activity in pyrazinamide (PZA)-resistant isolates is an important parameter to determine the susceptibility to pyrazinoic acid (PZA) because PZase converts PZA into pyrazinoic acid, which is protonated and becomes active against the mycobacterium. A nonfunctional PZase allows the mycobacterium to survive in the presence of PZA Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
gene pncA, genotyping, global statistics for 641 mutations in 171 (of 187) codons from 2760 resistant strains and 96 mutations from 3329 susceptible strains reported in 61 studies, overview Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information mutational statistics, especially mutations in the promoter region of pncA, Analysis of methods of phenotypic determination and genotype-phenotype evaluation for diagnostics in clinical drug studies and MIC determinations, overview. For diagnostics, individual mutations (or any subset) are not sufficiently sensitive. Assuming similar error profiles of the 5 phenotyping platforms included in the study, the entire enzyme and its promoter provide a combined estimated sensitivity of 83%. Molecular diagnostics of PZA resistance Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyrazinamide + H2O Mycobacterium tuberculosis
-
 pyrazinoic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyrazinamide + H2O
-
 Mycobacterium tuberculosis pyrazinoic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
PncA
-
 Mycobacterium tuberculosis
PZAse
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
malfunction a nonfunctional PZase in resistant strains allows the mycobacterium to survive in the presence of pyrazinamide. Alternative or complementary mechanism of resistance may exist Mycobacterium tuberculosis