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Literature summary for 3.5.1.96 extracted from

  • Guo, Y.; Lu, B.; Tang, H.; Bi, D.; Zhang, Z.; Lin, L.; Pang, H.
    Tolerance against butanol stress by disrupting succinylglutamate desuccinylase in Escherichia coli (2019), RSC Adv., 9, 11683-11695 .
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
gene astE, quantitative RT-PCR enzyme expression analysis Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of a butanol-tolerant Escherichia coli mutant strain BW25113-DELTAastE by disrupting gene astE (encoding succinylglutamate desuccinylase) to obtain higher butanol tolerance (increased by 34.6%). Analysis of the mechanism of increased tolerance to butanol in the mutant, overview. Mutant BW25113-DELTAastE mainly regulates intracellular pH-homeostasis to adapt to butanol stress, indicating the non-negligible impact of pH on microbial butanol tolerance, broadening our understanding of microbial butanol tolerance and providing a strategy for the rational engineering of a more robust butanol-producing host Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P76215
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Escherichia coli BW25113 P76215
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Synonyms

Synonyms Comment Organism
astE
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 Escherichia coli

General Information

General Information Comment Organism
malfunction mutant BW25113-DELTAastE mainly regulates intracellular pH-homeostasis to adapt to butanol stress, indicating the non-negligible impact of pH on microbial butanol tolerance, broadening our understanding of microbial butanol tolerance and providing a strategy for the rational engineering of a more robust butanol-producing host Escherichia coli
metabolism analysis of the mechanism of the enzyme's tolerance to butanol, metabolome and GC-MS-based transcriptomics analysis showing that acid-activated glutaminase ybaS and the amino acid antiporter gadC are significantly upregulated, but the levels of L-arginine and glutamate are not significantly increased and decreased, detailed overview Escherichia coli