Literature summary for 3.5.1.94 extracted from

Kurihara, S.; Oda, S.; Kumagai, H.; Suzuki, H.
gamma-Glutamyl-gamma-aminobutyrate hydrolase in the putrescine utilization pathway of Escherichia coli K-12 (2006), FEMS Microbiol. Lett., 256, 318-323.

Search for more literature for 3.5.1.94

General Stability

General Stability	Organism
stabilization of PuuD activity by beta-mercaptoethanol	Escherichia coli K-12

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28500	-	2 * 28500, calcaulation from amino-acid sequence	Escherichia coli K-12
57000	-	gel filtration	Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-(gamma-glutamylamino)butanoate + H2O	Escherichia coli K-12	enzyme is involved in the gamma-glutamyl-gamma-aminobutyrate hydrolase pathway for the degradation of putrescine to succinate semialdehyde. PuuD is essential for growth on putrescine as a sole source of nitrogen. Expression of the enzyme is induced by putrescine and O2. Addition of succinate and NH4Cl to the medium reduces the expression of PuuD	4-aminobutanoate + L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli K-12	P76038	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli K-12

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	enzyme is expressed most highly in early stationary phase. The expression of puuD is dramatically repressed when Escherichia coli is grown up with low aeration	Escherichia coli K-12	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-(gamma-glutamylamino)butanoate + H2O	enzyme is involved in the gamma-glutamyl-gamma-aminobutyrate hydrolase pathway for the degradation of putrescine to succinate semialdehyde. PuuD is essential for growth on putrescine as a sole source of nitrogen. Expression of the enzyme is induced by putrescine and O2. Addition of succinate and NH4Cl to the medium reduces the expression of PuuD	Escherichia coli K-12	4-aminobutanoate + L-glutamate	-	?
gamma-(glutamylamino)butanoate + H2O	Cys114 is the active center of PuuD	Escherichia coli K-12	gamma-aminobutanoate + L-glutamate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 28500, calcaulation from amino-acid sequence	Escherichia coli K-12

Synonyms

Synonyms	Comment	Organism
PuuD	-	Escherichia coli K-12

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	9	-	Escherichia coli K-12

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Release 2023.1 (January 2023)