Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.93 extracted from

  • Gaurav, K.; Kundu, K.; Kundu, S.
    Biosynthesis of cephalosporin-C acylase enzyme: optimal media design, purification, and characterization (2010), Artif. Cells Blood Substit. Immobil. Biotechnol., 38, 277-283.
    View publication on PubMed
Search for more literature for 3.5.1.93

Application

Application Comment Organism
biotechnology with an aim to increase the yield of 7-aminocephalosporanic acid production, a stepwise strategy, statistical medium is applied for optimizing the medium composition for the production of CPC acylase Micrococcus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 7-aminocephalosporanic acid estimated value: 9.43 mg/ml, pH 7.6, 34°C, Vmax: 7.65 U/ml Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
-
 Micrococcus luteus

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O
-
 Micrococcus luteus 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?

Synonyms

Synonyms Comment Organism
cephalosporin-C acylase
-
 Micrococcus luteus
CPC acylase
-
 Micrococcus luteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34
-
 for the 7-aminocephalosporanic acid production Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 for the 7-aminocephalosporanic acid production Micrococcus luteus