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Literature summary for 3.5.1.93 extracted from

  • Nupura, H.; Asmita, T.; Sharath, B.; Asmita, P.
    Media optimization for the production of cephalosporin C acylase from a novel bacterial source: Alcaligenes xylosoxidans MTCC *491 (2008), Res. J. Biotechnol., 3, 16-21.
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Search for more literature for 3.5.1.93

Activating Compound

Activating Compound Comment Organism Structure
D-glucose 9% increase of activity in the presence of glucose Achromobacter xylosoxidans
galactose 22% increase of activity in the presence of galactose Achromobacter xylosoxidans

Application

Application Comment Organism
industry great potential for industrial application of the lysis genes-assisted cell disruption Achromobacter xylosoxidans

Cloned(Commentary)

Cloned (Comment) Organism
a recombinant Escherichia coli strain simultaneously containing the maltose binding protein, the lysis genes and the target GLA gene in a same operon is constructed Achromobacter xylosoxidans

Inhibitors

Inhibitors Comment Organism Structure
additional information nitrogen sources have a repressive effect on enzyme activity Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O Achromobacter xylosoxidans
-
 (7R)-7-aminocephalosporanate + glutarate
-
 ?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O Achromobacter xylosoxidans MTCC *491
-
 (7R)-7-aminocephalosporanate + glutarate
-
 ?

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans
-
-
-
Achromobacter xylosoxidans MTCC *491
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 6810 U/l in a 5l fermentor Achromobacter xylosoxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
-
 Achromobacter xylosoxidans (7R)-7-aminocephalosporanate + glutarate
-
 ?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
-
 Achromobacter xylosoxidans MTCC *491 (7R)-7-aminocephalosporanate + glutarate
-
 ?
cephalosporin C + H2O
-
 Achromobacter xylosoxidans cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
cephalosporin C + H2O
-
 Achromobacter xylosoxidans MTCC *491 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?

Synonyms

Synonyms Comment Organism
CCA
-
 Achromobacter xylosoxidans
cephalosporin C acylase
-
 Achromobacter xylosoxidans
glutaryl-7-aminocephalosporanic acid acylase
-
 Achromobacter xylosoxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Achromobacter xylosoxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Achromobacter xylosoxidans