Application | Comment | Organism |
---|---|---|
synthesis | production of 7-aminocephalosporanate on a large scale at 25°C using enzyme immobilized by epoxide silanization | Pseudomonas sp. |
General Stability | Organism |
---|---|
the enzyme is thermostabilized by immobilization using epoxide silanization | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
recombinant | - |
Pseudomonas sp. KAC-1 | - |
recombinant | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. KAC-1 | 7-aminocephalosporanate + glutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GL-7-ACA acylase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
soluble enzyme and enzyme immobilized on silica gel modified by epoxide silanization | Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: soluble enzyme shows about 45% of maximal activity, enzyme immobilized on silica gel modified by epoxide silanization shows about 75% of maximal activity, 55°C: soluble enzyme shows about 40% of maximal activity, enzyme immobilized on silica gel modified by epoxide silanization shows about 55% of maximal activity | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
60 min, soluble enzyme and enzyme immobilized on silica gel modified by epoxide silanization lose about 10% of its activity | Pseudomonas sp. |
50 | - |
60 min, soluble enzyme loses 77% of its activity, enzyme immobilized on silica gel modified by epoxide silanization loses 60% of its activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | soluble enzyme and enzyme immobilized on silica gel modified by epoxide silanization | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: soluble enzyme shows about 35% of maximal activity, enzyme immobilized on silica gel modified by epoxide silanization shows about 65% of maximal activity, pH 10.0: soluble enzyme shows about 70% of maximal activity, enzyme immobilized on silica gel modified by epoxide silanization shows about 85% of maximal activity | Pseudomonas sp. |