Protein Variants | Comment | Organism |
---|---|---|
additional information | substitution of the first residue of the beta-subunit, Ser, results in a complete loss of enzyme activity, and substitution of the last residue of the spacer, Gly, leads to an inactive and unprocessed precursor | Pseudomonas sp. 130 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. 130 | O86089 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the gene encoding the enzyme is expressed as a precursor polypeptide consisting of a signal peptide followed by alpha- and beta-subunits, which are separated by a spacer peptide. Removing the signal peptide has little effect on precursor processing or enzyme activity. The precursor is supposed to be processed autocatalytically, probably intramolecularly | Pseudomonas sp. 130 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | low activity | Pseudomonas sp. 130 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl 7-aminodeacetoxycephalosporanic acid + H2O | - |
Pseudomonas sp. 130 | 7-aminodeacetoxycephalosporanic acid + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. 130 | 7-aminocephalosporanate + glutarate | - |
? | |
penicillin G + H2O | low activity | Pseudomonas sp. 130 | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |