Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli in a two-cistron system | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
L-gamma-glutamyl-p-nitroanilide | - |
Pseudomonas sp. | |
6.1 | - |
glutaryl-7-aminocephalosporanic acid | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
1 * 40000 + 1 * 22000, SDS-PAGE | Pseudomonas sp. |
40000 | - |
1 * 40000 + 1 * 22000, SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. V22 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.6 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. V22 | 7-aminocephalosporanate + glutarate | - |
? | |
L-gamma-glutamyl p-nitroanilide + H2O | - |
Pseudomonas sp. | L-glutamate + p-nitroaniline | - |
? | |
L-gamma-glutamyl p-nitroanilide + H2O | - |
Pseudomonas sp. V22 | L-glutamate + p-nitroaniline | - |
? | |
additional information | purified enzyme exhibits also gamma-glutamyltranspeptidase activity with L-gamma-glutamyl-p-nitroanilide | Pseudomonas sp. | ? | - |
? | |
additional information | purified enzyme exhibits also gamma-glutamyltranspeptidase activity with L-gamma-glutamyl-p-nitroanilide | Pseudomonas sp. V22 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 40000 + 1 * 22000, SDS-PAGE | Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | isoelectric focusing | - |
4.6 |