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Literature summary for 3.5.1.92 extracted from

  • Dupre, S.; Cavallini, D.
    Purification and properties of pantetheinase from horse kidney (1979), Methods Enzymol., 62, 262-267.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates Equus caballus
dithiothreitol activates Equus caballus

Inhibitors

Inhibitors Comment Organism Structure
cysteamine 5 mM, 55% inhibition Equus caballus
iodoacetamide at high concentrations Equus caballus
iodoacetate at high concentrations Equus caballus
NaN3 15 mM, 90% inhibition Equus caballus
pantothenate 50 mM, 60% inhibition Equus caballus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5
-
pantetheine
-
Equus caballus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
ultracentrifugation Equus caballus

Organism

Organism UniProt Comment Textmining
Equus caballus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Equus caballus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Equus caballus
-
kidney
-
Equus caballus
-
liver
-
Equus caballus
-
muscle
-
Equus caballus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
-
Equus caballus

Storage Stability

Storage Stability Organism
-20°C, pH 7, several months without significant loss of activity Equus caballus
0°C, dilute solutions lose 5% activity per week Equus caballus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-pantetheine + H2O
-
Equus caballus (R)-pantothenate + cysteamine
-
?
(R)-pantetheine 4-phosphate + H2O 10% of the activity with (R)-pantetheine Equus caballus ?
-
?

Synonyms

Synonyms Comment Organism
pantetheinase
-
Equus caballus
pantetheine hydrolase
-
Equus caballus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5.5
-
Equus caballus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7.6 good stability, rapid decrease outside this range Equus caballus

pI Value

Organism Comment pI Value Maximum pI Value
Equus caballus
-
-
4.8