Cloned (Comment) | Organism |
---|---|
gene nfdA, recombinant expression of the enzyme from vectors pESH19cF-nfdA, pESH19kF-nfdA, and pESH19aF-nfdA in Escherichia coli strain Stbl2 and Streptomyces lividans strain TK24 | Arthrobacter pascens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of six inducible shuttle vectors, pESH19cF, pESH19cR, pESH19kF, pESH19kR, pESH19aF, and pESH19aR, for Streptomyces-Escherichia coli, evaluation of stability and activity in expression of different enzymes including the N-substituted formamide deformylase as model enzymes, overview. Catechol 2,3-dioxygenase, nitrilase, and N-substituted formamide deformylase encoding genes are expressed from the different vectors as reporter genes showing that pESH19cF, pESH19kF, and pESH19aF possess inducible expression ability in Streptomyces lividans strain TK24. The stability test shows that the pSH19-derived shuttle vectors are stable in Escherichia coli strain Stbl2 and Streptomyces lividans strain TK24. Streptomyces lividans strain TK24 is the better host | Arthrobacter pascens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent on | Arthrobacter pascens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzylformamide + H2O | Arthrobacter pascens | - |
formate + benzylamine | - |
? | |
N-benzylformamide + H2O | Arthrobacter pascens F164 | - |
formate + benzylamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter pascens | Q68AP4 | - |
- |
Arthrobacter pascens F164 | Q68AP4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzylformamide + H2O | - |
Arthrobacter pascens | formate + benzylamine | - |
? | |
N-benzylformamide + H2O | - |
Arthrobacter pascens F164 | formate + benzylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NfdA | - |
Arthrobacter pascens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Arthrobacter pascens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arthrobacter pascens |