Literature summary for 3.5.1.91 extracted from

Fukatsu, H.; Herai, S.; Hashimoto, Y.; Maseda, H.; Higashibata, H.; Kobayashi, M.
High-level expression of a novel amine-synthesizing enzyme, N-substituted formamide deformylase, in Streptomyces with a strong protein expression system (2005), Protein Expr. Purif., 40, 212-219.

Search for more literature for 3.5.1.91

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Streptomyces lividans strain TK 24	Arthrobacter pascens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.067	-	N-benzylformamide	25°C, pH 7.5, recombinant enzyme	Arthrobacter pascens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58773	-	2 * 58773, MALDI-TOF mass spectroscopy	Arthrobacter pascens
61000	-	2 * 61000, SDS-PAGE	Arthrobacter pascens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-benzylformamide + H2O	Arthrobacter pascens	-	formate + benzylamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter pascens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, ammonium sulfate, DEAE-Sephacel, Resource ISO	Arthrobacter pascens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
28.8	-	-	Arthrobacter pascens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-benzylformamide + H2O	-	Arthrobacter pascens	formate + benzylamine	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 61000, SDS-PAGE	Arthrobacter pascens
dimer	2 * 58773, MALDI-TOF mass spectroscopy	Arthrobacter pascens

Synonyms

Synonyms	Comment	Organism
NfdA	-	Arthrobacter pascens

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Release 2023.1 (January 2023)