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Literature summary for 3.5.1.9 extracted from
- Tryptophan catabolism via kynurenine production in Streptomyces coelicolor: identification of three genes coding for the enzymes of tryptophan to anthranilate pathway (2012), Appl. Microbiol. Biotechnol., 94, 719-728.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene kynB or SCO3644, gene expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Streptomyces coelicolor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
N-formyl-L-kynurenine | pH 7.4, temperature not specified in the publication, recombinant His6-tagged enzyme | Streptomyces coelicolor |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required | Streptomyces coelicolor | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | Streptomyces coelicolor | - |
formate + L-kynurenine | - |
? | |
| N-formyl-L-kynurenine + H2O | Streptomyces coelicolor M145 | - |
formate + L-kynurenine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | Q9X8R1 | gene SCO3644; gene kynB or SCO3644 | - |
| Streptomyces coelicolor M145 | Q9X8R1 | gene SCO3644; gene kynB or SCO3644 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration | Streptomyces coelicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | - |
pH 7.4, temperature not specified in the publication, purified recombinant His6-tagged enzyme | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | - |
Streptomyces coelicolor | formate + L-kynurenine | - |
? | |
| N-formyl-L-kynurenine + H2O | - |
Streptomyces coelicolor M145 | formate + L-kynurenine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KFA | - |
Streptomyces coelicolor |
| kynurenine formamidase | - |
Streptomyces coelicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Streptomyces coelicolor |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Streptomyces coelicolor | tryptophan positively affects kynB transcription | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the tryptophan-to-anthranilate pathway | Streptomyces coelicolor |
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Release 2023.1 (January 2023)
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