Any feedback?
Literature summary for 3.5.1.9 extracted from
- Cloning, expression, and catalytic triad of recombinant arylformamidase (2005), Protein Expr. Purif., 44, 39-44.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and anlysis, expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH10B | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D247A | site-directed mutagenesis, inactive mutant | Mus musculus |
| H279A | site-directed mutagenesis, inactive mutant | Mus musculus |
| S162A | site-directed mutagenesis, inactive mutant | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
N-formyl-L-kynurenine | pH 7.4, native wild-type enzyme | Mus musculus |  |
| 0.19 | - |
N-formyl-L-kynurenine | pH 7.4, recombinant wild-type enzyme | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34229 | - |
x * 34229, sequence calculation | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | Mus musculus | - |
formate + L-kynurenine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q8K4H1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from liver cytosol, recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography, gel filtration, and nickel affinity chromatography, to high purity, recombinant wild-type enzyme 90fold | Mus musculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| N-formyl-L-kynurenine + H2O = formate + L-kynurenine | the catalytic triad is formed by residues Ser162, Asp247, and His279 | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42 | - |
purified recombinant wild-type enzyme | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | - |
Mus musculus | formate + L-kynurenine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34229, sequence calculation | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AFMID | - |
Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
