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Literature summary for 3.5.1.9 extracted from

  • Shinohara, R.; Ishiguro, I.
    New formamidase having substrate specificity for o-formylaminoacetophenone in pig liver (1977), Biochim. Biophys. Acta, 483, 409-415.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 76% inhibition at 0.1 mM Sus scrofa
diisopropylfluorophosphate complete inhibition at 0.01 mM Sus scrofa
Hg2+ 50% inhibition at 0.1 mM Sus scrofa
L-kynurenine strong non-competitive inhibition Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-formyl-o-aminoacetophenone + H2O Cavia porcellus
-
formate + o-aminoacetophenone
-
?
N-formyl-o-aminoacetophenone + H2O Oryctolagus cuniculus
-
formate + o-aminoacetophenone
-
?
N-formyl-o-aminoacetophenone + H2O Sus scrofa the only substrate formate + o-aminoacetophenone
-
?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
guinea pig, formamidase II
-
no activity in Mus musculus
-
not present in liver
-
no activity in Rattus sp.
-
not present in liver
-
Oryctolagus cuniculus
-
rabbit, formamidase II
-
Sus scrofa
-
formamidase II
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cavia porcellus
-
liver
-
Sus scrofa
-
liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.33
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formyl-o-aminoacetophenone + H2O
-
Cavia porcellus formate + o-aminoacetophenone
-
?
N-formyl-o-aminoacetophenone + H2O
-
Oryctolagus cuniculus formate + o-aminoacetophenone
-
?
N-formyl-o-aminoacetophenone + H2O the only substrate Sus scrofa formate + o-aminoacetophenone
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
Hg2+
-
Sus scrofa