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Literature summary for 3.5.1.9 extracted from

  • Serrano, A.E.; Nagayama, F.
    Purification and characterization of liver arylformamidase in rainbow trout and cattle (1991), Comp. Biochem. Physiol. B, 98, 381-387.
    View publication on PubMed
Search for more literature for 3.5.1.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 N-formyl-L-kynurenine
-
 Oncorhynchus mykiss
0.45
-
 N-formyl-DL-kynurenine
-
 Oncorhynchus mykiss
0.78
-
 N-formyl-L-kynurenine
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31700
-
 gel filtration Oncorhynchus mykiss
42600
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-formyl-L-kynurenine + H2O Bos taurus involved in the pathway of tryptophan degradation L-kynurenine + formate
-
 ?
N-formyl-L-kynurenine + H2O Oncorhynchus mykiss involved in the pathway of tryptophan degradation L-kynurenine + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Oncorhynchus mykiss
-
 rainbow trout
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Bos taurus
to homogeneity, chromatography techniques Oncorhynchus mykiss

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-
liver
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
425
-
-
 Bos taurus
1304
-
-
 Oncorhynchus mykiss

Storage Stability

Storage Stability Organism
4°C, 60% of activity recovered after seven months Bos taurus
4°C, fully active within seven months Oncorhynchus mykiss

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formyl-DL-kynurenine + H2O mixture of equal amounts of D- and L-kynurenine Bos taurus L-kynurenine + formate + N-formyl-D-kynurenine N-formyl-D-kynurenine might inhibit activity ?
N-formyl-DL-kynurenine + H2O mixture of equal amounts of D- and L-kynurenine Oncorhynchus mykiss L-kynurenine + formate + N-formyl-D-kynurenine N-formyl-D-kynurenine might inhibit activity ?
N-formyl-L-kynurenine + H2O involved in the pathway of tryptophan degradation Bos taurus L-kynurenine + formate
-
 ?
N-formyl-L-kynurenine + H2O involved in the pathway of tryptophan degradation Oncorhynchus mykiss L-kynurenine + formate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bos taurus
40
-
-
 Oncorhynchus mykiss

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 80% remaining activity after 30 min Bos taurus
60
-
 16% remaining activity after 30 min Oncorhynchus mykiss

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Oncorhynchus mykiss
8
-
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 high activity within Bos taurus
6.5 11 high activity within Oncorhynchus mykiss

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 4 no activity Oncorhynchus mykiss
3 4 remaining activity: 20-50% Bos taurus