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Literature summary for 3.5.1.89 extracted from

  • Ashraf, M.; Yadav, B.; Perinthottathil, S.; Kumar, K.S.; Vats, D.; Muthuswami, R.; Komath, S.S.
    N-Acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase from Entamoeba histolytica: metal alters catalytic rates but not substrate affinity (2011), J. Biol. Chem., 286, 2543-2549.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the cytoplasmic domain MBPEhDELTATMPIG-L is expressed in Escherichia coli Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
additional information treatment with EDTA consistently causes up to 2% reduction in activity, the activity is not significantly affected by treatment with 1,10-phenanthroline Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00195
-
N-acetyl-D-glucosaminylphosphatidylinositol in the absence of metal ions, in 50 mM acetate buffer at pH 5.5 and 37°C Entamoeba histolytica
0.00205
-
N-acetyl-D-glucosaminylphosphatidylinositol in the presence of 5 mM Mn2+, in 50 mM acetate buffer at pH 5.5 and 37°C Entamoeba histolytica

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the de-N-acetylase activity of the cyctoplasmic domain MBPEhDELTATMPIG-L is 2fold higher in the presence of 5 mM Co2+ Entamoeba histolytica
Mg2+ the de-N-acetylase activity of the cyctoplasmic domain MBPEhDELTATMPIG-L is 2fold higher in the presence of 5 mM Mg2+ Entamoeba histolytica
Mn2+ the de-N-acetylase activity of the cyctoplasmic domain MBPEhDELTATMPIG-L is 2fold higher in the presence of 5 mM Mn2+ Entamoeba histolytica
additional information 5 mM Zn2+ does not stimulate the de-N-acetylase activity of the cyctoplasmic domain MBPEhDELTATMPIG-L Entamoeba histolytica
additional information the protein is active even in the absence of metal. The metal ion does not directly participate in ligand binding or recognition but is important for the catalytic efficiency of the enzymatic site Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
x * 66000, cytoplasmic domain MBPEhDELTATMPIG-L, SDS-PAGE Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O Entamoeba histolytica
-
D-Glucosaminylphosphatidylinositol + acetate
-
?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O
-
Entamoeba histolytica D-Glucosaminylphosphatidylinositol + acetate
-
?

Subunits

Subunits Comment Organism
? x * 66000, cytoplasmic domain MBPEhDELTATMPIG-L, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
GlcNAc-PI-de-N-acetylase
-
Entamoeba histolytica
N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
-
Entamoeba histolytica
PIG-L protein
-
Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Entamoeba histolytica