Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.84 extracted from

  • Cameron, S.; Durchschein, K.; Richman, J.; Sadowsky, M.; Wackett, L.
    New family of biuret hydrolases involved in s-triazine ring metabolism (2011), ACS Catal., 1, 1075-1082.
No PubMed abstract available
Search for more literature for 3.5.1.84

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Rhizobium leguminosarum bv. viciae

General Stability

General Stability Organism
potassium phosphate buffer yields optimal catalytic rates Rhizobium leguminosarum bv. viciae

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide more than 90% loss of activity at 0.05 mM; more than 90% loss of activity at 0.5 mM Rhizobium leguminosarum bv. viciae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 Biuret in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the addition of ZnSO4 or CoSO4 (0.05 mM and 1 mM) to enzyme assays gives only nominal increases in activity. No metal cofactors are detected Rhizobium leguminosarum bv. viciae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27121
-
 x * 27121, calculated from amino acid sequence Rhizobium leguminosarum bv. viciae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biuret + H2O Rhizobium leguminosarum bv. viciae the enzyme acts uniquely on biuret physiologically allophanic acid + NH3
-
 ir

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum bv. viciae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Rhizobium leguminosarum bv. viciae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 below 0.02 micromol/min/mg, using (E)-diazene-1,2-dicarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 1,1-dimethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 1-carbamimidoylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 1-ethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 1-hydroxyurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 1-methylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 1-methylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 1-prop-2-en-1-ylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 2-cyanoacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 2-methylpropanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using 3-oxobutanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using acetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using butanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using dicarbonodithioimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using ethanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using ethanethioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using ethyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using ethyl carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using formamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using hexanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using hydrazinecarbothioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using hydrazinecarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using N-carbamothioylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using N-hydroxydicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using N-nitrodicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using prop-2-enamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using propanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using sulfuric diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using thiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.02
-
 below 0.02 micromol/min/mg, using urea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.04
-
 using N-carbamoylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
0.17
-
 using 1-formylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
6.08
-
 using dicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
8.9
-
 using biuret as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-diazene-1,2-dicarboxamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
1,1-dimethylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
1-carbamimidoylthiourea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
1-ethylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
1-formylurea + H2O
-
 Rhizobium leguminosarum bv. viciae ?
-
 ?
1-formylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
1-hydroxyurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
1-methylthiourea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
1-methylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
1-prop-2-en-1-ylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
2-cyanoacetamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
2-methylpropanamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3-oxobutanamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
acetamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
biuret + H2O the enzyme acts uniquely on biuret physiologically Rhizobium leguminosarum bv. viciae allophanic acid + NH3
-
 ir
butanediamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
carbamoylcarbamate + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
dicarbonimidic diamide + H2O
-
 Rhizobium leguminosarum bv. viciae ?
-
 ?
dicarbonimidic diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
dicarbonodithioimidic diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
ethanediamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
ethanethioamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
ethyl acetate + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
ethyl carbamoylcarbamate + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
formamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
hexanediamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
hydrazinecarbothioamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
hydrazinecarboxamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
N-carbamothioylacetamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
N-carbamoylacetamide + H2O
-
 Rhizobium leguminosarum bv. viciae ?
-
 ?
N-carbamoylacetamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
N-hydroxydicarbonimidic diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
N-nitrodicarbonimidic diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
prop-2-enamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
propanediamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
sulfuric diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
thiourea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
urea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27121, calculated from amino acid sequence Rhizobium leguminosarum bv. viciae

Synonyms

Synonyms Comment Organism
biuret hydrolase
-
 Rhizobium leguminosarum bv. viciae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
 Biuret in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Rhizobium leguminosarum bv. viciae

pH Range

pH Minimum pH Maximum Comment Organism
4.5 11 about 50% activity at pH 4.5 and pH 11.0 Rhizobium leguminosarum bv. viciae

pI Value

Organism Comment pI Value Maximum pI Value
Rhizobium leguminosarum bv. viciae calculated from amino acid sequence
-
 5.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
170
-
 Biuret in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae