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Literature summary for 3.5.1.84 extracted from

  • Cook, A.M.; Beilstein, P.; Grossenbacher, H.; Hutter, R.
    Ring cleavage and degradative pathway of cyanuric acid in bacteria (1985), Biochem. J., 231, 25-30.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.84

General Stability

General Stability Organism
strain D, bovine serum albumin, large amounts, stabilizes, rapid loss of activity can be decreased to 25% with large amounts of bovine serum albumin Pseudomonas sp.
strain D, dithiothreitol, 10 mM, stabilizes, rapid loss of activity can be decreased to 25% with 10 mM dithiothreitol Pseudomonas sp.
strain D, enzyme very labile during purification Pseudomonas sp.
strain D, enzyme very labile, complete loss of activity in a few hours Pseudomonas sp.
strain D, glycerol, 1.5 M, stabilizes, rapid loss of activity can be decreased to 25% with 1.5 M glycerol Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
DEAE-cellulose strain D, slow inactivation, inactivation accelerated markedly on addition of dithiothreitol Pseudomonas sp.
dithiothreitol strain D, accelerates inactivation by DEAE-cellulose markedly Pseudomonas sp.
Tris strain D, inactivation Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biuret + H2O Pseudomonas sp. imidodicarbonic diamide urea + CO2 + NH3
-
 ?
biuret + H2O Klebsiella pneumoniae imidodicarbonic diamide urea + CO2 + NH3
-
 ?
biuret + H2O Pseudomonas sp. involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine urea + CO2 + NH3
-
 ?
biuret + H2O Klebsiella pneumoniae involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine urea + CO2 + NH3
-
 ?
biuret + H2O Pseudomonas sp. involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria urea + CO2 + NH3
-
 ?
biuret + H2O Klebsiella pneumoniae involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria urea + CO2 + NH3
-
 ?
biuret + H2O Pseudomonas sp. second enzyme of degradative pathway of cyanuric acid urea + CO2 + NH3
-
 ?
biuret + H2O Klebsiella pneumoniae second enzyme of degradative pathway of cyanuric acid urea + CO2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae 99
-
-
-
Pseudomonas sp.
-
 strain D, NRRLB-12228 and strain A, NRRLB-12227
-

Purification (Commentary)

Purification (Comment) Organism
partial, strain D, NRRLB-12228, enzyme very labile during purification Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.
additional information
-
-
 Klebsiella pneumoniae

Storage Stability

Storage Stability Organism
strain D, rapid complete loss of activity in a few hours Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biuret + H2O imidodicarbonic diamide Pseudomonas sp. urea + CO2 + NH3
-
 ?
biuret + H2O imidodicarbonic diamide Klebsiella pneumoniae urea + CO2 + NH3
-
 ?
biuret + H2O involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Pseudomonas sp. urea + CO2 + NH3
-
 ?
biuret + H2O involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine Klebsiella pneumoniae urea + CO2 + NH3
-
 ?
biuret + H2O involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Pseudomonas sp. urea + CO2 + NH3
-
 ?
biuret + H2O involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria Klebsiella pneumoniae urea + CO2 + NH3
-
 ?
biuret + H2O second enzyme of degradative pathway of cyanuric acid Pseudomonas sp. urea + CO2 + NH3
-
 ?
biuret + H2O second enzyme of degradative pathway of cyanuric acid Klebsiella pneumoniae urea + CO2 + NH3
-
 ?

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 strain D, essentially inactive at pH 9.5 Pseudomonas sp.