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Literature summary for 3.5.1.82 extracted from
- Molecular chaperones facilitate the soluble expression of N-acyl-D-amino acid amidohydrolases in Escherichia coli (2004), J. Ind. Microbiol. Biotechnol., 31, 421-426.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of active D-AGase in Escherichia coli strain JM109 or strain BL21 partially in inclusion bodies, co-expression of chaperones GroELS, DnaKJE, and trigger factor TF | Achromobacter xylosoxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter xylosoxidans | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38.1 | - |
soluble recombinant enzyme from expression without chaperones and trigger factor | Achromobacter xylosoxidans |
| 95.8 | - |
soluble recombinant enzyme from co-expression with chaperones and trigger factor | Achromobacter xylosoxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is specific for N-acyl-D-glutamate | Achromobacter xylosoxidans | ? | - |
? |  |
| additional information | the enzyme is specific for N-acyl-D-glutamate | Achromobacter xylosoxidans A-6 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-AGase | - |
Achromobacter xylosoxidans |
| N-acyl-D-glutamate amidohydrolase | - |
Achromobacter xylosoxidans |
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Release 2023.1 (January 2023)
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