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Literature summary for 3.5.1.82 extracted from

  • Yoshimune, K.; Hirayama, A.; Moriguchi, M.
    A metal ion as a cofactor attenuates substrate inhibition in the enzymatic production of a high concentration of D-glutamate using N-acyl-D-glutamate amidohydrolase (2005), Biotechnol. Lett., 27, 1325-1328.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is used for production of D-glutamate, Co2+ might be a useful additive for stabilization of the enzyme Achromobacter xylosoxidans

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of D-AGase in Escherichia coli Achromobacter xylosoxidans

General Stability

General Stability Organism
Co2+ stabilizes Achromobacter xylosoxidans

Inhibitors

Inhibitors Comment Organism Structure
N-acetyl-DL-glutamate substrate inhibition at high concentration, 94% inhibition at 1 M, the inhibition is reduced by Co2+ Achromobacter xylosoxidans

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ stabilizes partially at 1 mM Achromobacter xylosoxidans
Co2+ stabilizes, optimal at 1 mM, no activation of enzymic activity Achromobacter xylosoxidans
Mg2+ stabilizes partially at 1 mM Achromobacter xylosoxidans
additional information effects on enzyme stability by metal ions, overview Achromobacter xylosoxidans
Zn2+ required, D-AGase is a zinc-metalloenzyme, stabilizes partially, no activation of enzymic activity Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-glutamate + H2O Achromobacter xylosoxidans
-
acetate + D-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Achromobacter xylosoxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glutamate + H2O
-
Achromobacter xylosoxidans acetate + D-glutamate
-
?

Synonyms

Synonyms Comment Organism
D-AGase
-
Achromobacter xylosoxidans
N-acyl-D-glutamate amidohydrolase
-
Achromobacter xylosoxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Achromobacter xylosoxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Achromobacter xylosoxidans