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Literature summary for 3.5.1.81 extracted from

  • Lai, W.L.; Chou, L.Y.; Ting, C.Y.; Kirby, R.; Tsai, Y.C.; Wang, A.H.; Liaw, S.H.
    The functional role of the binuclear metal center in D-aminoacylase: one-metal activation and second-metal attenuation (2004), J. Biol. Chem., 279, 13962-13967.
    View publication on PubMed
Search for more literature for 3.5.1.81

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the inactive H220A mutant displays that the endogenous Zn2+ shifts to alpha3 subsite coordinated by His67, His69, Cys96 and Asp366 Alcaligenes faecalis

Protein Variants

Protein Variants Comment Organism
C96D inactive mutant enzymes Alcaligenes faecalis
D366A inactive mutant enzymes Alcaligenes faecalis
H220A inactive mutant enzymes Alcaligenes faecalis

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ noncompetitive, binds at the alpha1 subsite ligated by His67, His69 and Asp366 Alcaligenes faecalis
Cu2+ noncompetitive, binds at the alpha2 subsite chelated by His67, His69 and Cys96 Alcaligenes faecalis
additional information no inhibition by Ni2+, Co2+, Mg2+, Mn2+ and Ca2+ Alcaligenes faecalis
Zn2+ noncompetitive, binds at the alpha1 subsite ligated by His67, His69 and Asp366 Alcaligenes faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
 N-Acetyl-D-Met wild-type enzyme Alcaligenes faecalis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ catalytically essential Zn2+ is tightly bound at the beta site with coordination by Cys96, His220 and His250 Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis Q9AGH8
-
-
Alcaligenes faecalis DA1 Q9AGH8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetyl-D-Met + H2O
-
 Alcaligenes faecalis acetate + D-Met
-
 ?
N-Acetyl-D-Met + H2O
-
 Alcaligenes faecalis DA1 acetate + D-Met
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
438
-
 N-Acetyl-D-Met wild-type enzyme Alcaligenes faecalis