Any feedback?
Literature summary for 3.5.1.81 extracted from
- Characterization of D-aminoacylase from Alcaligenes denitrificans DA181 (1992), Biosci. Biotechnol. Biochem., 56, 1392-1395.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | partial | Achromobacter denitrificans |  |
| Cu2+ | 1 mM, complete inhibition | Achromobacter denitrificans | |
| EDTA | activity is fully restored by Co2+, and partially restored by Zn2+ | Achromobacter denitrificans | |
| Pb2+ | partial | Achromobacter denitrificans | |
| phosphate | - |
Achromobacter denitrificans | |
| Zn2+ | 1 mM, complete inhibition | Achromobacter denitrificans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
N-Chloroacetyl-D-Val | - |
Achromobacter denitrificans | |
| 0.14 | - |
N-Acetyl-D-Leu | - |
Achromobacter denitrificans | |
| 0.27 | - |
N-Acetyl-D-Phe | - |
Achromobacter denitrificans | |
| 0.48 | - |
N-Acetyl-D-Met | - |
Achromobacter denitrificans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | the enzyme contains 2.1 gatom of zinc per mol of enzyme | Achromobacter denitrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Achromobacter denitrificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter denitrificans | - |
basonym Alcaligenes denitrificans, strain DA 181 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-D-Leu + H2O | - |
Achromobacter denitrificans | acetate + D-Leu | - |
? | |
| N-Acetyl-D-Met + H2O | - |
Achromobacter denitrificans | acetate + D-Met | - |
? | |
| N-Acetyl-D-Phe + H2O | - |
Achromobacter denitrificans | acetate + D-Phe | - |
? | |
| N-Chloroacetyl-D-Val + H2O | - |
Achromobacter denitrificans | Chloroacetate + D-Val | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Achromobacter denitrificans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
in presence of 0.2 mg/ml bovine serum albumin, 1 h, stable up to | Achromobacter denitrificans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 382 | - |
N-Chloroacetyl-D-Val | - |
Achromobacter denitrificans | |
| 468 | - |
N-Acetyl-D-Leu | - |
Achromobacter denitrificans | |
| 877 | - |
N-Acetyl-D-Phe | - |
Achromobacter denitrificans | |
| 1040 | - |
N-Acetyl-D-Met | - |
Achromobacter denitrificans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Achromobacter denitrificans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | stable | Achromobacter denitrificans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
