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Literature summary for 3.5.1.81 extracted from

  • Moriguchi, M.; Sakai, K.; Miyamoto, Y.; Wakayama, M.
    Production, purification, and characterization of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (1993), Biosci. Biotechnol. Biochem., 57, 1149-1152.
    View publication on PubMed
Search for more literature for 3.5.1.81

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Achromobacter xylosoxidans
Cu2+
-
 Achromobacter xylosoxidans
D-Phe
-
 Achromobacter xylosoxidans
Fe2+
-
 Achromobacter xylosoxidans
N-Acetyl-D-allosoleucine
-
 Achromobacter xylosoxidans
Ni2+
-
 Achromobacter xylosoxidans
Zn2+
-
 Achromobacter xylosoxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.8
-
 N-Acetyl-D-Leu
-
 Achromobacter xylosoxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 1 * 52000, SDS-PAGE Achromobacter xylosoxidans
58000
-
 gel filtration Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Achromobacter xylosoxidans the best inducers are a poor substrate, N-acetyl-gamma-methyl-D-Leu, and an inhibitor, N-acetyl-D-alloisoleucine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Achromobacter xylosoxidans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
990
-
-
 Achromobacter xylosoxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the best inducers are a poor substrate, N-acetyl-gamma-methyl-D-Leu, and an inhibitor, N-acetyl-D-alloisoleucine Achromobacter xylosoxidans ?
-
 ?
N-acetyl-D-Leu + H2O
-
 Achromobacter xylosoxidans acetate + D-Leu
-
 ?
N-Acetyl-D-Met + H2O
-
 Achromobacter xylosoxidans acetate + D-Met
-
 ?
N-Acetyl-D-norleucine + H2O
-
 Achromobacter xylosoxidans acetate + D-norleucine
-
 ?
N-Acetyl-D-Phe + H2O
-
 Achromobacter xylosoxidans acetate + D-Phe
-
 ?
N-Butyl-D-Leu + H2O
-
 Achromobacter xylosoxidans Butanoate + D-Leu
-
 ?
N-Formyl-D-Leu + H2O
-
 Achromobacter xylosoxidans Formate + D-Leu
-
 ?
N-Propionyl-D-Leu + H2O
-
 Achromobacter xylosoxidans Propanoate + D-Leu
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Achromobacter xylosoxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Achromobacter xylosoxidans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Achromobacter xylosoxidans
50
-
 pH 8.1, 10 min, optimal stability at Achromobacter xylosoxidans
50
-
 pH 7.4, 10 min, 5% loss of activity Achromobacter xylosoxidans
50
-
 pH 8.3, 10 min, 17% loss of activity Achromobacter xylosoxidans
60
-
 complete loss of activity above Achromobacter xylosoxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Achromobacter xylosoxidans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
 50°C, 10 min, 5% loss of activity Achromobacter xylosoxidans
8.1
-
 50°C, 10 min, optimal stability at Achromobacter xylosoxidans
8.3
-
 50°C, 10 min, 17% loss of activity Achromobacter xylosoxidans