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Literature summary for 3.5.1.81 extracted from
- Purification and properties of D-aminoacylase of Streptomyces olivaceus (1978), Agric. Biol. Chem., 42, 107-133.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| CoCl2 protects from denaturation | Streptomyces olivaceus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Streptomyces olivaceus |  |
| Cu2+ | - |
Streptomyces olivaceus | |
| EDTA | - |
Streptomyces olivaceus | |
| Fe2+ | weak | Streptomyces olivaceus | |
| Hg2+ | - |
Streptomyces olivaceus | |
| Ni2+ | weak | Streptomyces olivaceus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.13 | - |
N-Acetyl-D-phenylglycine | - |
Streptomyces olivaceus | |
| 1.71 | - |
N-Acetyl-D-Phe | - |
Streptomyces olivaceus | |
| 2.43 | - |
N-Acetyl-D-Leu | - |
Streptomyces olivaceus | |
| 2.9 | - |
N-Acetyl-D-Trp | - |
Streptomyces olivaceus | |
| 2.95 | - |
N-Acetyl-D-Phe | - |
Streptomyces olivaceus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activation | Streptomyces olivaceus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces olivaceus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Streptomyces olivaceus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| 60 min preincubation with 1 mM Na2EDTA at 30°C causes irreversible inactivation | Streptomyces olivaceus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 571.1 | - |
- |
Streptomyces olivaceus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-D-Leu + H2O | - |
Streptomyces olivaceus | acetate + D-Leu | - |
? | |
| N-Acetyl-D-Phe + H2O | - |
Streptomyces olivaceus | acetate + D-Phe | - |
? | |
| N-Acetyl-D-phenylglycine + H2O | - |
Streptomyces olivaceus | acetate + D-phenylglycine | - |
? | |
| N-Acetyl-D-Trp + H2O | - |
Streptomyces olivaceus | acetate + D-Trp | - |
? | |
| N-acetyl-D-Val + H2O | - |
Streptomyces olivaceus | acetate + D-Val | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Streptomyces olivaceus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 50% of maximal activity, 60°C: about 30% of maximal activity | Streptomyces olivaceus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 7.0, 15 min, stable up to | Streptomyces olivaceus |
| 60 | - |
pH 7.0, 15 min, complete inactivation | Streptomyces olivaceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptomyces olivaceus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 9.3 | pH 6.3: about 30% of maximal activity, pH 9.3: about 70% of maximal activity | Streptomyces olivaceus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
5°C, 20 h, complete loss of activity | Streptomyces olivaceus |
| 5.5 | - |
5°C, 20 h, about 50% loss of activity | Streptomyces olivaceus |
| 6 | - |
5°C, 20 h, stable | Streptomyces olivaceus |
| 7.5 | - |
5°C, 20 h, about 40% loss of activity | Streptomyces olivaceus |
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