Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
M167L/M169L | residual activity after treatment with 0.5 mM H2O2 for 15 min is 37% for mutant enzyme, compared to 21% for wild-type enzyme | Agrobacterium tumefaciens |
M184L | residual activity after treatment with 0.5 mM H2O2 for 15 min is 57% for mutant enzyme, compared to 21% for wild-type enzyme | Agrobacterium tumefaciens |
M220L | residual activity after treatment with 0.5 mM H2O2 for 15 min is 62% for mutant enzyme, compared to 21% for wild-type enzyme | Agrobacterium tumefaciens |
M239L | mutant enzyme is stable after treatment with 0.5 mM H2O2 for 15 min compared to 79% loss of wild-type activity | Agrobacterium tumefaciens |
M239L/M244L | mutant enzyme is stable after treatment with 0.5 mM H2O2 for 15 min compared to 79% loss of wild-type activity | Agrobacterium tumefaciens |
M244L | mutant enzyme is stable after treatment with 0.5 mM H2O2 for 15 min compared to 79% loss of wild-type activity | Agrobacterium tumefaciens |
M31L | residual activity after treatment with 0.5 mM H2O2 for 15 min is 8% for mutant enzyme, compared to 21% for wild-type enzyme | Agrobacterium tumefaciens |
M5L | residual activity after treatment with 0.5 mM H2O2 for 15 min is 51% for mutant enzyme, compared to 21% for wild-type enzyme | Agrobacterium tumefaciens |
M73L | residual activity after treatment with 0.5 mM H2O2 for 15 min is 84% for mutant enzyme, compared to 21% for wild-type enzyme | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Oxidation Stability | Organism |
---|---|
residual activity after treatment with 0.5 mM H2O2 for 15 min is 21% for wild-type enzyme, 51% for mutant enzyme M5L, 8% for mutant enzyme M31L, 84% for mutant enzyme M73L, 37% for mutant enzyme M167L/M169L, 57% for mutant enzymeM184L, 62% for mutant enzyme M220L. The mutant enzymes M239L, M244L and M239L/M244L are stable to treatment with 0.5 mM H2O2 for 15 min | Agrobacterium tumefaciens |
Purification (Comment) | Organism |
---|---|
- |
Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-D-p-hydroxyphenylglycine + H2O | - |
Agrobacterium tumefaciens | D-p-hydroxyphenylglycine + NH3 + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-NCAase | - |
Agrobacterium tumefaciens |