Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.77 extracted from

  • Chien, H.C.R.; Hsu, C.L.; Hu, H.Y.; Wang, W.C.; Hsu, W.H.
    Enhancing oxidative resistance of Agrobacterium radiobacter N-carbamoyl D-amino acid amidohydrolase by engineering solvent-accessible methionine residues (2002), Biochem. Biophys. Res. Commun., 297, 282-287.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
M167L/M169L residual activity after treatment with 0.5 mM H2O2 for 15 min is 37% for mutant enzyme, compared to 21% for wild-type enzyme Agrobacterium tumefaciens
M184L residual activity after treatment with 0.5 mM H2O2 for 15 min is 57% for mutant enzyme, compared to 21% for wild-type enzyme Agrobacterium tumefaciens
M220L residual activity after treatment with 0.5 mM H2O2 for 15 min is 62% for mutant enzyme, compared to 21% for wild-type enzyme Agrobacterium tumefaciens
M239L mutant enzyme is stable after treatment with 0.5 mM H2O2 for 15 min compared to 79% loss of wild-type activity Agrobacterium tumefaciens
M239L/M244L mutant enzyme is stable after treatment with 0.5 mM H2O2 for 15 min compared to 79% loss of wild-type activity Agrobacterium tumefaciens
M244L mutant enzyme is stable after treatment with 0.5 mM H2O2 for 15 min compared to 79% loss of wild-type activity Agrobacterium tumefaciens
M31L residual activity after treatment with 0.5 mM H2O2 for 15 min is 8% for mutant enzyme, compared to 21% for wild-type enzyme Agrobacterium tumefaciens
M5L residual activity after treatment with 0.5 mM H2O2 for 15 min is 51% for mutant enzyme, compared to 21% for wild-type enzyme Agrobacterium tumefaciens
M73L residual activity after treatment with 0.5 mM H2O2 for 15 min is 84% for mutant enzyme, compared to 21% for wild-type enzyme Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-

Oxidation Stability

Oxidation Stability Organism
residual activity after treatment with 0.5 mM H2O2 for 15 min is 21% for wild-type enzyme, 51% for mutant enzyme M5L, 8% for mutant enzyme M31L, 84% for mutant enzyme M73L, 37% for mutant enzyme M167L/M169L, 57% for mutant enzymeM184L, 62% for mutant enzyme M220L. The mutant enzymes M239L, M244L and M239L/M244L are stable to treatment with 0.5 mM H2O2 for 15 min Agrobacterium tumefaciens

Purification (Commentary)

Purification (Comment) Organism
-
Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
Agrobacterium tumefaciens D-p-hydroxyphenylglycine + NH3 + CO2
-
?

Synonyms

Synonyms Comment Organism
D-NCAase
-
Agrobacterium tumefaciens