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Literature summary for 3.5.1.75 extracted from

  • Akutsu-Shigeno, Y.; Adachi, Y.; Yamada, C.; Toyoshima, K.; Nomura, N.; Uchiyama, H.; Nakajima-Kambe, T.
    Isolation of a bacterium that degrades urethane compounds and characterization of its urethane hydrolase (2006), Appl. Microbiol. Biotechnol., 70, 422-429.
    View publication on PubMed
Search for more literature for 3.5.1.75

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is strongly induced by acetanilide, effect of carbon sources and inducers on cell growth, overview Rhodococcus equi

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Rhodococcus equi

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 37% inhibition at 1 mM Rhodococcus equi
Cd2+ 18% inhibition at 1 mM Rhodococcus equi
Co2+ 11% inhibition at 1 mM Rhodococcus equi
Cu2+ 61% inhibition at 1 mM Rhodococcus equi
Hg2+ 98% inhibition at 1 mM Rhodococcus equi
additional information no or poor inhibition by Mn2+, EDTA, 8-hydroxyquinoline, DTT, and iodoacetamide Rhodococcus equi
Ni2+ 32% inhibition at 1 mM Rhodococcus equi
PMSF 84% inhibition at 1 mM Rhodococcus equi
Zn2+ 21% inhibition at 1 mM Rhodococcus equi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 4-nitrophenyl acetate pH 7.0, 30°C Rhodococcus equi
0.07
-
 ethyl N-phenylcarbamate pH 7.0, 30°C Rhodococcus equi
0.1
-
 4-nitrophenylbutyrate pH 7.0, 30°C Rhodococcus equi
0.13
-
 acetanilide pH 7.0, 30°C Rhodococcus equi
0.15
-
 4-nitroacetanilide pH 7.0, 30°C Rhodococcus equi
2.49
-
 L-alanine 4-nitroanilide pH 7.0, 30°C Rhodococcus equi
2.5
-
 butyramide pH 7.0, 30°C Rhodococcus equi
6.59
-
 ethyl carbamate pH 7.0, 30°C Rhodococcus equi
9.4
-
 phenyl acetamide pH 7.0, 30°C Rhodococcus equi
11.8
-
 Benzamide pH 7.0, 30°C Rhodococcus equi
49
-
 acetamide pH 7.0, 30°C Rhodococcus equi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20% activation at 1 mM Rhodococcus equi
NEM 12% activation at 1 mM Rhodococcus equi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 approximately, gel filtration Rhodococcus equi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hexamethylene dicarbamic acid dibutyl ester + H2O Rhodococcus equi
-
 ?
-
 ?
methylene bisphenyl dicarbamic acid dibutyl ester + H2O Rhodococcus equi
-
 ?
-
 ?
additional information Rhodococcus equi the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters ?
-
 ?
additional information Rhodococcus equi TB-60 the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters ?
-
 ?
toluene-2,4-dicarbamic acid dibutyl ester + H2O Rhodococcus equi
-
 toluene diamine + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus equi
-
 isolated from mineral medium
-
Rhodococcus equi TB-60
-
 isolated from mineral medium
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography Rhodococcus equi

Source Tissue

Source Tissue Comment Organism Textmining
additional information effect of carbon sources and inducers on cell growth, overview Rhodococcus equi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhodococcus equi
6
-
 purified native enzyme Rhodococcus equi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitroacetanilide + H2O
-
 Rhodococcus equi ?
-
 ?
4-nitrophenyl acetate + H2O
-
 Rhodococcus equi 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Rhodococcus equi TB-60 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Rhodococcus equi ?
-
 ?
acetamide + H2O
-
 Rhodococcus equi ?
-
 ?
acetamide + H2O
-
 Rhodococcus equi TB-60 ?
-
 ?
acetanilide + H2O
-
 Rhodococcus equi ?
-
 ?
benzamide + H2O
-
 Rhodococcus equi ?
-
 ?
benzamide + H2O
-
 Rhodococcus equi TB-60 ?
-
 ?
butyl carbamate + H2O
-
 Rhodococcus equi CO2 + NH3 + butanol
-
 ?
butyramide + H2O
-
 Rhodococcus equi ?
-
 ?
ethyl carbamate + H2O
-
 Rhodococcus equi CO2 + NH3 + ethanol
-
 ?
ethyl N-phenylcarbamate + H2O
-
 Rhodococcus equi CO2 + aniline + ethanol
-
 ?
hexamethylene dicarbamic acid dibutyl ester + H2O
-
 Rhodococcus equi ?
-
 ?
hexamethylene dicarbamic acid dibutyl ester + H2O
-
 Rhodococcus equi ? product identification and quantification, overview ?
L-alanine-4-nitroanilide + H2O
-
 Rhodococcus equi ?
-
 ?
methylene bisphenyl dicarbamic acid dibutyl ester + H2O
-
 Rhodococcus equi ?
-
 ?
methylene bisphenyl dicarbamic acid dibutyl ester + H2O
-
 Rhodococcus equi ? product identification and quantification, overview ?
additional information the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters Rhodococcus equi ?
-
 ?
additional information substrate specificity, overview, no activity with L-leucine-4-nitroanilide, methyl benzoate, and ethyl benzoate Rhodococcus equi ?
-
 ?
additional information the bacterium that degrades aliphatic and aromatic urethane compounds, it also hydrolyzes anilides, amides, and esters Rhodococcus equi TB-60 ?
-
 ?
additional information substrate specificity, overview, no activity with L-leucine-4-nitroanilide, methyl benzoate, and ethyl benzoate Rhodococcus equi TB-60 ?
-
 ?
phenyl acetamide + H2O
-
 Rhodococcus equi ?
-
 ?
toluene-2,4-dicarbamic acid dibutyl ester + H2O
-
 Rhodococcus equi toluene diamine + ?
-
 ?
toluene-2,4-dicarbamic acid dibutyl ester + H2O
-
 Rhodococcus equi toluene diamine + ? product identification and quantification, overview ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Rhodococcus equi

Synonyms

Synonyms Comment Organism
urethane hydrolase
-
 Rhodococcus equi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Rhodococcus equi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60
-
 Rhodococcus equi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 rapid inactivation at pH 4.0, more slowly inactivation at 60°C Rhodococcus equi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Rhodococcus equi

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 stable Rhodococcus equi