Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 0.4 M, enhances activity of unpurified enzyme by 55%, but no effect with purified enzyme | Hordeum vulgare | |
K2SO4 | 0.4 M, enhances activity of unpurified enzyme by 36% | Hordeum vulgare |
General Stability | Organism |
---|---|
ammonium sulfate, 0.4 M, stabilizes during fractionation | Hordeum vulgare |
glycerol, 10%, stabilizes | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | - |
Hordeum vulgare | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-feruloylglycine + H2O | Hordeum vulgare | best substrate | ferulate + glycine | - |
? | |
N-feruloylglycine + H2O | Hordeum vulgare | enzyme increases in activity during the first 14 h, maximum activity, of germination: enzyme plays a role during germination | ferulate + glycine | - |
? | |
N-feruloylglycine + H2O | Hordeum vulgare | may be responsible for the hydrolysis of N-feruloylglycyl-moieties of certain reserve proteins, because such N-feruloylglycine-containing proteins occur in barley seeds | ferulate + glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley | - |
Hordeum vulgare | - |
cv Zephyr and cv Gitane | - |
Lactuca sativa | - |
lettuce, very low activity | - |
Pisum sativum | - |
pea, very low activity | - |
Secale cereale | - |
rye, low activity | - |
Spinacia oleracea | - |
spinach, very low activity | - |
Trifolium sp. | - |
clover, very low activity | - |
Purification (Comment) | Organism |
---|---|
from germinated and excised embryos | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aleurone layer | - |
Hordeum vulgare | - |
embryo | - |
Pisum sativum | - |
embryo | - |
Secale cereale | - |
embryo | highest activity in germinated barley embryos | Hordeum vulgare | - |
leaf | - |
Spinacia oleracea | - |
leaf | - |
Lactuca sativa | - |
leaf | - |
Trifolium sp. | - |
additional information | tissue distribution | Hordeum vulgare | - |
additional information | tissue distribution | Spinacia oleracea | - |
additional information | tissue distribution | Pisum sativum | - |
additional information | tissue distribution | Lactuca sativa | - |
additional information | tissue distribution | Secale cereale | - |
additional information | tissue distribution | Trifolium sp. | - |
additional information | no activity in endosperm, shoots and roots | Hordeum vulgare | - |
seed | - |
Hordeum vulgare | - |
stem | - |
Trifolium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Spinacia oleracea |
additional information | - |
- |
Pisum sativum |
additional information | - |
- |
Lactuca sativa |
additional information | - |
- |
Secale cereale |
additional information | - |
- |
Trifolium sp. |
additional information | - |
highest activity in cv Gitane, almost twice the activity of cv Zephyr | Hordeum vulgare |
0.022 | - |
- |
Hordeum vulgare |
Storage Stability | Organism |
---|---|
-28°C, stable for several months without serious loss of activity | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with N-acetylglycine | Hordeum vulgare | ? | - |
? | |
N-4-hydroxybenzoylglycine + H2O | poorly hydrolyzed | Hordeum vulgare | 4-hydroxybenzoate + glycine | - |
? | |
N-feruloylglycine + H2O | - |
Spinacia oleracea | ferulate + glycine | - |
? | |
N-feruloylglycine + H2O | - |
Pisum sativum | ferulate + glycine | - |
? | |
N-feruloylglycine + H2O | - |
Lactuca sativa | ferulate + glycine | - |
? | |
N-feruloylglycine + H2O | - |
Secale cereale | ferulate + glycine | - |
? | |
N-feruloylglycine + H2O | - |
Trifolium sp. | ferulate + glycine | - |
? | |
N-feruloylglycine + H2O | best substrate | Hordeum vulgare | ferulate + glycine | - |
? | |
N-feruloylglycine + H2O | enzyme increases in activity during the first 14 h, maximum activity, of germination: enzyme plays a role during germination | Hordeum vulgare | ferulate + glycine | - |
? | |
N-feruloylglycine + H2O | may be responsible for the hydrolysis of N-feruloylglycyl-moieties of certain reserve proteins, because such N-feruloylglycine-containing proteins occur in barley seeds | Hordeum vulgare | ferulate + glycine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-FGAH | - |
Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hordeum vulgare |
32 | - |
- |
Hordeum vulgare |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable up to, unstable above | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Hordeum vulgare |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
unstable below | Hordeum vulgare |
7.5 | 10 | very stable between | Hordeum vulgare |