General Stability | Organism |
---|---|
bivalent cations important for stability | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | o-phenanthroline, 10 mM: 97% inhibition, partially reversed by CuCl2, CoCl2 and to a lesser extent by ZnCl2 and MnCl2, 1 mM: 29% inhibition, 0.1 mM: no inhibition. 2.5 mM: 73% inhibition, totally reversed by 0.66 mM ZnCl2, CuCl2 or CoCl2 and to a lesser extent also by nickel and manganous ions | Hordeum vulgare | |
1,4-dithiothreitol | 2 mM: 100% inhibition | Hordeum vulgare | |
2-Iodosobenzoate | 5 mM: 100% inhibition | Hordeum vulgare | |
2-mercaptoethanol | 20 mM: 100% inhibition | Hordeum vulgare | |
Co2+ | 1 mM: about 37% inhibition | Hordeum vulgare | |
CoSO4 | 1 mM: about 37% inhibition | Hordeum vulgare | |
diphenyl-1,5-thiocarbazone | dithizone, 10 mM: 79% inhibition | Hordeum vulgare | |
EDTA | 10 mM: 61% inhibition | Hordeum vulgare | |
glutathione | reduced glutathione, 50 mM: 100% inhibition | Hordeum vulgare | |
HgCl2 | 0.005 mM: 100% inhibition | Hordeum vulgare | |
iodoacetamide | 7.5 mM: 100% inhibition | Hordeum vulgare | |
iodoacetate | 4 mM: 100% inhibition | Hordeum vulgare | |
L-cysteine | 50 mM: 100% inhibition | Hordeum vulgare | |
Metal chelating reagents | high concentration, 10 mM | Hordeum vulgare | |
additional information | kinetics of inhibition; no inhibition by cystine and disodium maleate | Hordeum vulgare | |
N-ethylmaleimide | 1 mM: 100% inhibition | Hordeum vulgare | |
N-feruloyl-Gly-L-LeuOH | 5 mM: potent reversible inhibitor, kinetics of inhibition. Ki: 0.3 mM | Hordeum vulgare | |
N-feruloyl-Gly-L-PheOH | 5 mM: potent reversible inhibitor, kinetics of inhibition. Ki: 0.042 mM | Hordeum vulgare | |
N-feruloyldipeptides | potent reversible inhibitor | Hordeum vulgare | |
p-chloromercuribenzoate | 0.05 mM: 100% inhibition | Hordeum vulgare | |
sulfhydryl reagents | - |
Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data, kinetic studies | Hordeum vulgare | |
0.084 | - |
N-feruloylglycine | Eadie-Hofstee plot | Hordeum vulgare | |
0.085 | - |
N-feruloylglycine | - |
Hordeum vulgare | |
0.086 | - |
N-feruloylglycine | Lineweaver-Burk plot | Hordeum vulgare | |
0.133 | - |
N-Sinapoylglycine | - |
Hordeum vulgare | |
0.217 | - |
N-Feruloyl-L-alanine | - |
Hordeum vulgare | |
0.385 | - |
N-p-Coumaroylglycine | - |
Hordeum vulgare | |
0.426 | - |
N-cinnamoylglycine | - |
Hordeum vulgare | |
0.833 | - |
N-Dihydroferuloylglycine | - |
Hordeum vulgare | |
0.909 | - |
N-Feruloyl-D,L-aspartic acid | - |
Hordeum vulgare | |
1.11 | - |
N-Vanilloyl-1-glycine | - |
Hordeum vulgare | |
1.18 | - |
N-4-Hydroxybenzoylglycine | - |
Hordeum vulgare | |
2 | - |
N-benzoylglycine | - |
Hordeum vulgare |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Bivalent cations | important for proper function | Hordeum vulgare | |
additional information | no activation by Co2+, 1 mM CoSO4 even inhibitory | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-feruloylglycine + H2O | Hordeum vulgare | best substrate | ferulate + glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley | - |
Hordeum vulgare | - |
cv Zephyr | - |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N-feruloylglycine + H2O = ferulate + glycine | thermodynamic data | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Hordeum vulgare | - |
seed | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Hordeum vulgare |
0.022 | - |
- |
Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity | Hordeum vulgare | ? | - |
? | |
additional information | N-syringoylglycine is only poorly hydrolyzed, N-galloylglycine, N-protocatechuoylglycine and N-caffeoylglycine are hydrolyzed, but due to oxidation and other reactions the activity of the enzyme with the corresponding substrate cannot be accurately measured | Hordeum vulgare | ? | - |
? | |
additional information | no activity with N-acetylamino acids, Azocoll, N-carbobenzoxy-L-Phe-L-AlaOH, Nalpha-benzoyl-L-Arg-ethyl ester, N-feruloyl-Gly-L-PheOH, N-feruloyl-Gly-L-LeuOH, L-Leu-p-nitroanilide, Nalpha-benzoyl-D,L-Arg-p-nitroanilide, N-L-leucyl-L-tyrosine, N-L-alanyl-L-proline, N-L-phenylalanylglycine, N-L-phenylalanyl-L-alanine, N-formyl-amino acids, N-formyl-L-aspartic acid, N-formyl-L-methionine and N-feruloylamide | Hordeum vulgare | ? | - |
? | |
additional information | no deformylase or amidase activity | Hordeum vulgare | ? | - |
? | |
additional information | hydrolyses range of L-amino acids from the cinnamoyl, substituted cinnamoyl and benzoyl series | Hordeum vulgare | ? | - |
? | |
N-4-hydroxybenzoylglycine + H2O | - |
Hordeum vulgare | 4-hydroxybenzoate + glycine | - |
? | |
N-benzoylglycine + H2O | - |
Hordeum vulgare | benzoate + glycine | - |
? | |
N-cinnamoylglycine + H2O | - |
Hordeum vulgare | cinnamate + glycine | - |
? | |
N-dihydroferuloylglycine + H2O | - |
Hordeum vulgare | dihydroferulate + glycine | - |
? | |
N-feruloyl-D,L-aspartic acid + H2O | - |
Hordeum vulgare | ferulate + D,L-aspartate | - |
? | |
N-feruloyl-L-alanine + H2O | - |
Hordeum vulgare | ferulate + L-alanine | - |
? | |
N-feruloylglycine + H2O | best substrate | Hordeum vulgare | ferulate + glycine | - |
? | |
N-p-coumaroylglycine + H2O | - |
Hordeum vulgare | p-coumarate + glycine | - |
? | |
N-sinapoylglycine + H2O | - |
Hordeum vulgare | sinapate + glycine | - |
? | |
N-syringoylglycine + H2O | only poorly hydrolyzed | Hordeum vulgare | syringate + glycine | - |
? | |
N-vanilloyl-1-glycine + H2O | - |
Hordeum vulgare | 4-hydroxy-3-methoxybenzoate + glycine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-FGAH | - |
Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Hordeum vulgare |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
N-feruloyl-Gly-L-PheOH | 5 mM: potent reversible inhibitor, kinetics of inhibition. | Hordeum vulgare | |
0.3 | - |
N-feruloyl-Gly-L-LeuOH | 5 mM: potent reversible inhibitor, kinetics of inhibition | Hordeum vulgare |