Literature summary for 3.5.1.69 extracted from

Li, Y.; Arigi, E.; Eichert, H.; Levery, S.
Mass spectrometry of fluorocarbon-labeled glycosphingolipids (2010), J. Mass Spectrom., 45, 504-519.

Search for more literature for 3.5.1.69

Application

Application	Comment	Organism
synthesis	a method for generation of novel fluorocarbon derivatives of glycosphingolipids has been developed. SCDase is used to remove the fatty acid from the ceramide moiety, after which a fluorocarbon-rich substituent is incorporated at the free amine of the sphingoid	Pseudomonas sp. TK4

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. TK4	-	commercial preparation	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Pseudomonas sp. TK4	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	purified neutral glycosphingolipids and gangliosides from bovine brain tissue are treated with SCDase and derivatized by fluorocarbon-rich substituent tags. The products are recovered by FluorosTM solid phase extraction	Pseudomonas sp. TK4	?	the products are recovered by FluorosTM solid ohase extraction and analyzed by MALDI-QIT-TOF mass spectrometry and ESI-LIT mass spectrometry	?

Synonyms

Synonyms	Comment	Organism
SCDase	-	Pseudomonas sp. TK4
sphingolipid ceramide N-deacylase	-	Pseudomonas sp. TK4

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Release 2023.1 (January 2023)