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Literature summary for 3.5.1.65 extracted from

  • Soda, K.; Uchiyama, K.; Ogata, K.
    Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part I. Bacterial and enzymatic hydrolysis of L- and D-isomers of theanine and identification of products (1966), Agric. Biol. Chem., 30, 541-546.
No PubMed abstract available
Search for more literature for 3.5.1.65

Activating Compound

Activating Compound Comment Organism Structure
D-glutamate activation and induction Pseudomonas aeruginosa
L-glutamate activation and induction Pseudomonas aeruginosa
L-Theanine activation and induction Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N5-ethyl-L-glutamine + H2O Pseudomonas aeruginosa N-ethyl-gamma-L-glutamine L-glutamate + ethylamine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas fluorescens
-
-
-
Pseudomonas sp.
-
 T-1
-
Pseudomonas sp. T-1
-
 T-1
-

Purification (Commentary)

Purification (Comment) Organism
partial, glutaminase could not be separated Pseudomonas aeruginosa

Reaction

Reaction Comment Organism Reaction ID
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine stoichiometry of reaction Pseudomonas fluorescens
a
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine stoichiometry of reaction Pseudomonas aeruginosa
a
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine stoichiometry of reaction Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N5-ethyl-L-glutamine + H2O
-
 Pseudomonas fluorescens L-glutamate + ethylamine
-
 ?
N5-ethyl-L-glutamine + H2O
-
 Pseudomonas aeruginosa L-glutamate + ethylamine
-
 ?
N5-ethyl-L-glutamine + H2O
-
 Pseudomonas sp. L-glutamate + ethylamine
-
 ?
N5-ethyl-L-glutamine + H2O N-ethyl-gamma-L-glutamine Pseudomonas aeruginosa L-glutamate + ethylamine
-
 ?
N5-ethyl-L-glutamine + H2O
-
 Pseudomonas sp. T-1 L-glutamate + ethylamine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas fluorescens
37
-
 assay at Pseudomonas aeruginosa
37
-
 assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas fluorescens
8
-
 assay at Pseudomonas aeruginosa
8
-
 assay at Pseudomonas sp.