Crystallization (Comment) | Organism |
---|---|
crystal structures of wild-type beta-alanine synthase in complex with the reaction product beta-alanine, and of the mutant E159A with the substrate N-carbamyl-beta-alanine | Lachancea kluyveri |
Protein Variants | Comment | Organism |
---|---|---|
E159A | 0.09% of the wild-type activity | Lachancea kluyveri |
E159D | 0.09% of the wild-type activity | Lachancea kluyveri |
H226E | no activity | Lachancea kluyveri |
H262A | 8.9% of the wild-type activity | Lachancea kluyveri |
H397N | 7.2% of the wild-type activity | Lachancea kluyveri |
R322A | 0.14% of the wild-type activity | Lachancea kluyveri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
N-Carbamoyl-beta-alanine | wild-type enzyme | Lachancea kluyveri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lachancea kluyveri | Q96W94 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-beta-alanine + H2O | - |
Lachancea kluyveri | beta-alanine + CO2 + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-alanine synthase | - |
Lachancea kluyveri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
N-Carbamoyl-beta-alanine | mutant enzyme E159A | Lachancea kluyveri | |
0.005 | - |
N-Carbamoyl-beta-alanine | mutant enzyme E159D | Lachancea kluyveri | |
0.0077 | - |
N-Carbamoyl-beta-alanine | mutant enzyme R322A | Lachancea kluyveri | |
0.38 | - |
N-Carbamoyl-beta-alanine | mutant enzyme H397N | Lachancea kluyveri | |
0.47 | - |
N-Carbamoyl-beta-alanine | mutant enzyme H262A | Lachancea kluyveri | |
5.3 | - |
N-Carbamoyl-beta-alanine | wild-type enzyme | Lachancea kluyveri |