General Stability | Organism |
---|---|
the hexamer is a stable enzyme species, detergents, e.g. 3% v/v CHAPSO or CHAPS, and reducing thiols stabilize purified enzyme in solution, 1 mM dithiothreitol stabilizes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-methyl-beta-alanine | product inhibition, the presence of product leads to dissociation of hexamers to inactive trimers | Rattus norvegicus | |
beta-Alanine | product inhibition, the presence of product leads to dissociation of hexamers to inactive trimers | Rattus norvegicus | |
gamma-aminobutyrate | - |
Rattus norvegicus | |
additional information | removal of the enzyme-bound zinc by chelators leads to loss of activity | Rattus norvegicus | |
propionate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
2-methyl-N-carbamoyl-beta-alanine | - |
Rattus norvegicus | |
0.008 | - |
N-Carbamoyl-beta-alanine | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains two tightly bound Zn2+, removal leads to loss of activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
12 * 42000, active dodecamer in the presence of substrate, SDS-PAGE | Rattus norvegicus |
42000 | - |
3 * 42000, inactive trimer in the presence of the product, SDS-PAGE | Rattus norvegicus |
42000 | - |
6 * 42000, the native enzyme is a hexamer, which readily associates to an active dodecamer in the presence of substrate and dissociates to an inactive trimer in the presence of the product, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-N-carbamoyl-beta-alanine + H2O | Rattus norvegicus | completes the catabolism of the pyrimidine bases uracil and thymine | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-beta-alanine + H2O | Rattus norvegicus | completes the catabolism of the pyrimidine bases uracil and thymine | beta-alanine + CO2 + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1096fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.88 | - |
N-carbamoyl-beta-alanine as substrate | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, buffer without stabilizing detergents and reducing thiols, 26 days, almost complete loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-N-carbamoyl-beta-alanine + H2O | - |
Rattus norvegicus | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
2-methyl-N-carbamoyl-beta-alanine + H2O | completes the catabolism of the pyrimidine bases uracil and thymine | Rattus norvegicus | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
additional information | enzyme shows cooperativity for the substrate and allosteric regulation | Rattus norvegicus | ? | - |
? | |
N-carbamoyl-beta-alanine + H2O | - |
Rattus norvegicus | beta-alanine + CO2 + NH3 | - |
? | |
N-carbamoyl-beta-alanine + H2O | completes the catabolism of the pyrimidine bases uracil and thymine | Rattus norvegicus | beta-alanine + CO2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 42000, active dodecamer in the presence of substrate, SDS-PAGE | Rattus norvegicus |
hexamer | 6 * 42000, the native enzyme is a hexamer, which readily associates to an active dodecamer in the presence of substrate and dissociates to an inactive trimer in the presence of the product, SDS-PAGE | Rattus norvegicus |
trimer | 3 * 42000, inactive trimer in the presence of the product, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
heating destabilizes | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
N-Carbamoyl-beta-alanine | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
propionate | - |
Rattus norvegicus | |
1.1 | - |
beta-Alanine | - |
Rattus norvegicus | |
1.6 | - |
gamma-aminobutyrate | - |
Rattus norvegicus | |
3.9 | - |
2-methyl-beta-alanine | - |
Rattus norvegicus |