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Literature summary for 3.5.1.6 extracted from
- beta-Alanine synthase, an enzyme involved in catabolism of uracil and thymine (2000), Methods Enzymol., 324, 399-410.
General Stability
| General Stability | Organism |
|---|---|
| the hexamer is a stable enzyme species, detergents, e.g. 3% v/v CHAPSO or CHAPS, and reducing thiols stabilize purified enzyme in solution, 1 mM dithiothreitol stabilizes | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-methyl-beta-alanine | product inhibition, the presence of product leads to dissociation of hexamers to inactive trimers | Rattus norvegicus |  |
| beta-Alanine | product inhibition, the presence of product leads to dissociation of hexamers to inactive trimers | Rattus norvegicus | |
| gamma-aminobutyrate | - |
Rattus norvegicus | |
| additional information | removal of the enzyme-bound zinc by chelators leads to loss of activity | Rattus norvegicus | |
| propionate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
2-methyl-N-carbamoyl-beta-alanine | - |
Rattus norvegicus | |
| 0.008 | - |
N-Carbamoyl-beta-alanine | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | contains two tightly bound Zn2+, removal leads to loss of activity | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
12 * 42000, active dodecamer in the presence of substrate, SDS-PAGE | Rattus norvegicus |
| 42000 | - |
3 * 42000, inactive trimer in the presence of the product, SDS-PAGE | Rattus norvegicus |
| 42000 | - |
6 * 42000, the native enzyme is a hexamer, which readily associates to an active dodecamer in the presence of substrate and dissociates to an inactive trimer in the presence of the product, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methyl-N-carbamoyl-beta-alanine + H2O | Rattus norvegicus | completes the catabolism of the pyrimidine bases uracil and thymine | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
| N-carbamoyl-beta-alanine + H2O | Rattus norvegicus | completes the catabolism of the pyrimidine bases uracil and thymine | beta-alanine + CO2 + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1096fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.88 | - |
N-carbamoyl-beta-alanine as substrate | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, buffer without stabilizing detergents and reducing thiols, 26 days, almost complete loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methyl-N-carbamoyl-beta-alanine + H2O | - |
Rattus norvegicus | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
| 2-methyl-N-carbamoyl-beta-alanine + H2O | completes the catabolism of the pyrimidine bases uracil and thymine | Rattus norvegicus | 2-methyl-beta-alanine + CO2 + NH3 | - |
? | |
| additional information | enzyme shows cooperativity for the substrate and allosteric regulation | Rattus norvegicus | ? | - |
? | |
| N-carbamoyl-beta-alanine + H2O | - |
Rattus norvegicus | beta-alanine + CO2 + NH3 | - |
? | |
| N-carbamoyl-beta-alanine + H2O | completes the catabolism of the pyrimidine bases uracil and thymine | Rattus norvegicus | beta-alanine + CO2 + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | 12 * 42000, active dodecamer in the presence of substrate, SDS-PAGE | Rattus norvegicus |
| hexamer | 6 * 42000, the native enzyme is a hexamer, which readily associates to an active dodecamer in the presence of substrate and dissociates to an inactive trimer in the presence of the product, SDS-PAGE | Rattus norvegicus |
| trimer | 3 * 42000, inactive trimer in the presence of the product, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heating destabilizes | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
N-Carbamoyl-beta-alanine | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
propionate | - |
Rattus norvegicus | |
| 1.1 | - |
beta-Alanine | - |
Rattus norvegicus | |
| 1.6 | - |
gamma-aminobutyrate | - |
Rattus norvegicus | |
| 3.9 | - |
2-methyl-beta-alanine | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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