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Literature summary for 3.5.1.55 extracted from

  • Murase, H.; Kunieda, T.; Terao, J.; Nagao, A.
    Purification and characterisation of long chain acyl aminoacylase from Penicillium sp. B-001 (1993), Biosci. Biotechnol. Biochem., 57, 854-855.
No PubMed abstract available
Search for more literature for 3.5.1.55

Inhibitors

Inhibitors Comment Organism Structure
DTT
-
 Penicillium sp.
Hg2+ 95% inhibition Penicillium sp.
PCMB
-
 Penicillium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 N-oleoyl-L-glutamate
-
 Penicillium sp.
0.21
-
 N-oleolyl-L-glutamate
-
 Penicillium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Penicillium sp.
Cd2+ activates Penicillium sp.
Cu2+ activates Penicillium sp.
Mg2+ activates Penicillium sp.
Ni2+ activates Penicillium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
192000
-
 gel filtration Penicillium sp.

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
-
-
Penicillium sp. B-001
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium sp.

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Penicillium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
624.5
-
-
 Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: acetyl-L-glutamate Penicillium sp. ?
-
 ?
additional information not: acetyl-L-glutamate Penicillium sp. B-001 ?
-
 ?
N-arachidonoyl-L-glutamate + H2O 32% of the activity with N-decanoyl-L-glutamate Penicillium sp. arachidonate + L-glutamate
-
 ?
N-benzoyl-L-glutamate + H2O 74% of the activity with N-decanoyl-L-glutamate Penicillium sp. benzoate + L-glutamate
-
 ?
N-decanoyl-L-glutamate + H2O best substrate Penicillium sp. decanoate + L-glutamate
-
 ?
N-decanoyl-L-glutamate + H2O best substrate Penicillium sp. B-001 decanoate + L-glutamate
-
 ?
N-lauroyl-L-glutamate + H2O 88% of the activity with N-decanoyl-L-glutamate Penicillium sp. lauroate + L-glutamate
-
 ?
N-lauroyl-L-glutamate + H2O 88% of the activity with N-decanoyl-L-glutamate Penicillium sp. B-001 lauroate + L-glutamate
-
 ?
N-myristoyl-L-glutamate + H2O
-
 Penicillium sp. myristoate + L-glutamate
-
 ?
N-myristoyl-L-glutamate + H2O
-
 Penicillium sp. B-001 myristoate + L-glutamate
-
 ?
N-octanoyl-L-glutamate + H2O 80% of the activity with decanoyl-L-glutamate Penicillium sp. octanoate + L-glutamate
-
 ?
N-oleolyl-L-glutamate + H2O
-
 Penicillium sp. oleate + L-glutamate
-
 ?
N-oleoyl-L-glutamate + H2O 67% of the activity with N-decanoyl-L-glutamate Penicillium sp. oleate + L-glutamate
-
 ?
N-palmitoyl-L-glutamate + H2O 49% of the activity with N-decanoyl-L-glutamate Penicillium sp. palmitate + L-glutamate
-
 ?
N-palmitoyl-L-glutamate + H2O 49% of the activity with N-decanoyl-L-glutamate Penicillium sp. B-001 palmitate + L-glutamate
-
 ?
N-stearoyl-L-glutamate + H2O 67% of the activity with N-decanoyl-L-glutamate Penicillium sp. stearate + L-glutamate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 8.0, 15 min, stable Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Penicillium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 30°C, 5 h, stable Penicillium sp.