Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.52 extracted from

  • Wang, S.; Wang, P.G.; Qi, Q.
    Influence of substrate conformation on the deglycosylation of ribonuclease B by recombinant yeast peptide:N-glycanase (2007), Acta Biochim. Biophys. Sin. (Shanghai), 39, 8-14.
    View publication on PubMed
Search for more literature for 3.5.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as inclusion bodies Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
EDTA activity is not restored by adding metal ions Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc metalloenzyme Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonuclease B + H2O When ribonuclease B is denatured at 60-65ºC or by 40-60 mM dithiothreitol, its deglycosylation by Png1p is most prominent Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
peptide:N-glycanase
-
 Saccharomyces cerevisiae
Png1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 37 20°C: about 60% of maximal activity, 37°C: about 55% of maximal activity Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5: about 50% of maximal activity, pH 10.0: about 70% of maximal activity Saccharomyces cerevisiae