Literature summary for 3.5.1.52 extracted from

Allen, M.D.; Buchberger, A.; Bycroft, M.
The PUB domain functions as a p97 binding module in human peptide N-glycanase (2006), J. Biol. Chem., 281, 25502-25508.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant enzyme PUB domain, comprising residues 11-109, fused to the lipoyl domain of Bacillus stearothermophilus dehihydrolipoamide acetyltransferase in Escherichia coli strain C41(DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified wild-type PUB domain, also as selenomethionine variants, by sitting drop vapour diffusion method at 17°C, with a reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris, pH 8.5, 30% PEG 4000, using 20% glycerol as cryoprotectant, X-ray diffraction structure determination and analysis at 1.6 A resolution, mutant L66M/L75M/L87M PUB domain are crystallized in 50% PEG 400, 0.1 M CHES, pH 9.5, 0.2 M NaCl, molecular replacement, X-ray diffraction structure determination and analysis at 1.9 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified wild-type PUB domain, also as selenomethionine variants, by sitting drop vapour diffusion method at 17°C, with a reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris, pH 8.5, 30% PEG 4000, using 20% glycerol as cryoprotectant, X-ray diffraction structure determination and analysis at 1.6 A resolution, mutant L66M/L75M/L87M PUB domain are crystallized in 50% PEG 400, 0.1 M CHES, pH 9.5, 0.2 M NaCl, molecular replacement, X-ray diffraction structure determination and analysis at 1.9 A resolution

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
L66M/L75M/L87M	site-directed mutagenesis, the PUB domain mutant shows slightly reduced p97 binding	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the enzyme deglycosylates misfolded glycoproteins, the enzyme is a mediator for p97 functions, the PUB domain functions as a p97 binding module in human enzyme, p97 is an AAA ATPase with an ubiquitin-selective molecular machine involved in multiple cellular processes including protein degradation through the ubiquitin-proteasome system	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzyme fusion PUB domain from Escherichia coli strain C41(DE3) by nickel affinity chromatography, ion exchange chromatography, and gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme deglycosylates misfolded glycoproteins, the enzyme is a mediator for p97 functions, the PUB domain functions as a p97 binding module in human enzyme, p97 is an AAA ATPase with an ubiquitin-selective molecular machine involved in multiple cellular processes including protein degradation through the ubiquitin-proteasome system	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	the PUB domain, comprising residues 11-109, functions as a p97 binding module in human enzyme, three-dimensional structure of the PUB domain	Homo sapiens

Synonyms

Synonyms	Comment	Organism
peptide N-glycanase	-	Homo sapiens